Authors:
Daniil OlennikovRussian Academy of Sciences Laboratory of Medical and Biological Researches, Department of Biologically Active Substances, Institute of General and Experimental Biology, Siberian Division Sakh’yanovoy st. 6 670047 Ulan-Ude Russian Federation

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Vyacheslav PartilkhaevRussian Academy of Sciences Laboratory of Medical and Biological Researches, Department of Biologically Active Substances, Institute of General and Experimental Biology, Siberian Division Sakh’yanovoy st. 6 670047 Ulan-Ude Russian Federation

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Athin-layer chromatographic (TLC) method was used for the quantification of four dominant flavonoids (rutin, narcissin, nicotiflorin, isoquercitrin) in the shoots of Cargana spinosa (L.) DC. (Fabaceae). Chromatographic separation was performed on silica gel HPTLC plates with double development of ethylacetate-1,2-dichloroethane- acetic acid-85% formic acid-water 10:2.5:1:1:0.8 (v/v) as mobile phase. The plates were scanned densitometrically at 360 and 387 nm. The method was validated for precision, repeatability, and accuracy. It was found to be precise: intraday and interday RSD were 1.48–1.87% and 1.59–1.97%, respectively. Instrumental precision and repeatability for the method were found to be 0.51–0.64 and 1.18–1.32 (CV (%)), respectively. Accuracy was checked by measuring the recovery at four levels; the average recovery was 98.19–101.36% for rutin, 98.04–101.14% for narcissin, 98.16–100.54% for nicotiflorin, and 98.11–101.98% for isoquercitrin. The method was used for the analysis of the flavonoids mentioned in C. spinosa shoots samples. Nicotiflorin was detected in Caragana genus for the first time.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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