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Piotr Migas Medical University of Gdańsk Department of Pharmacognosy with Medicinal Plant Garden Gen. J. Haller Al. 107 80-416 Gdańsk Poland

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Magdalena Heyka Medical University of Gdańsk Department of Pharmacognosy with Medicinal Plant Garden Gen. J. Haller Al. 107 80-416 Gdańsk Poland

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Loretta Pobłocka-Olech Medical University of Gdańsk Department of Pharmacognosy with Medicinal Plant Garden Gen. J. Haller Al. 107 80-416 Gdańsk Poland

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Mirosława Krauze-Baranowska Medical University of Gdańsk Department of Pharmacognosy with Medicinal Plant Garden Gen. J. Haller Al. 107 80-416 Gdańsk Poland

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Three step bivariant multiple development thin-layer chromatography (BMD-TLC) technique was used in the quantitative analysis of chelerithrine, berberine, and chelidonine in extracts of greater celandine herb, roots, and herbal drugs. The separation was carried out on 10 cm × 10 cm TLC Si60 F254s glass plates in the horizontal DS-Chamber. First, the alkaloids were separated at a distance of 6 cm into two zones using toluene-ethyl acetate-methanol (1:1:1 ν/ν) solvent mixture as mobile phase. The plate was cut at 4.5 cm into two parts, lower part containing berberine and chelerithrine and upper part with chelidonine zone. The separation of berberine and chelerithrine was continued using n-propanol-formic acid-water (84:8:8 ν/ν) as mobile phase at a distance of 4.5 cm. The separation of chelidonine from accompanied compounds was achieved using the ethyl acetate-formic acid-water (7:1:1 ν/ν) solvent mixture at a distance of 5 cm. The densitometric quantification of Chelidonium alkaloids was performed at λ = 290 nm. The test of linearity, precision, repeatability, limit of detection (LOD), limit of quantitation (LOQ), and recovery proved that the optimized BMD-TLC separation is useful for both qualitative and quantitative analysis of chelerithrine, berberine, and chelidonine in plant material and herbal drugs. Moreover, the quantitative analysis of these alkaloids in two commercial available herbal drugs (Iberogast and Enterosol) showed that their quantity in single dose is too low to induce hepatotoxicity even during long-term use.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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