JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 26: Issue 4
A rapid validated high-performance thin-layer chromatographic-densitometric method for the simultaneous estimation of different chemical-nature compounds piperine and gallic acid in pharmaceutical dosage forms
Authors:
Subrata De RMD Research and Development Center Waghaldhara Valsad 396375 India

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Pankaj Nariya RMD Research and Development Center Waghaldhara Valsad 396375 India

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Nikhil Jirankalgikar RMD Research and Development Center Waghaldhara Valsad 396375 India

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Pages:
325–330
Online Publication Date:
26 Jul 2013
Publication Date:
01 Aug 2013
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.26.2013.4.6
Keywords:
HPTLC; Densitometric; Method development and validation; Piperine; Gallic acid; Ayurvedic formulations
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Piperine and gallic acid are of different chemical natures — piperine is an alkaloid, while gallic acid a phenolic compound. They are used as marker compounds in many plant-based formulations. A highperformance thin-layer chromatographic (HPTLC)-densitometric method has been developed and validated for the simultaneous quantification of piperine and gallic acid as such and in pharmaceutical dosage forms. Toluene-ethyl acetate (3:7) was used as mobile phase and scanning was done at 254 and 340 nm. The method was validated with respect to linearity, reproducibility, specificity, accuracy, precision, robustness, and ruggedness. Both compounds showed good linearities in the range of 250–1750 ng. LOD and LOQ for piperine were 9.98 and 33.29 ng, while for gallic acid 25 and 83.33 ng. Average % RSD values of precision for piperine and gallic acid were 0.46% and 0.72%, respectively. % Recovery was 96–103%. The method is accurate, reproducible, cost-effective, and can be used in routine analysis.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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