JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 26: Issue 4
Validated high-performance thin-layer chromatographic method for the quantification of betulinic acid from two Indian plants of the species Dillenia and Ziziphus
Authors:
Dipal Gandhi Nirma University Department of Pharmacognosy, Institute of Pharmacy Ahmedabad 382 481 India

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Priti Mehta Nirma University Department of Pharmacognosy, Institute of Pharmacy Ahmedabad 382 481 India

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Pages:
331–335
Online Publication Date:
26 Jul 2013
Publication Date:
01 Aug 2013
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.26.2013.4.7
Keywords:
Betulinic acid; HPTLC
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Betulinic acid (BA), a potent anticancer agent, occurs in more than 50 plant species worldwide, out of which Dillenia and Ziziphus species are found in Gujarat, India. The presented research work was aimed at the development of a single validated high-performance thin-layer chromatographic (HPTLC) method which can quantify BA from different plant species and thereof from different chemical environments. The analysis was performed on a thin-layer chromatographic (TLC) precoated silica gel 60 F254 plate using a mobile phase of toluene-chloroform-ethanol (4:4:1, v/v/v) and after derivatization with anisaldehyde sulfuric acid reagent showed magenta color spot at RF 0.71. Densitometric evaluation of BA was carried out at 525 nm. The suitability of this HPTLC method for quantitative determination of BA was proved by validation in accordance with the requirements of the International Conference on Harmonization (ICH) guidelines. The occurrence of BA is more frequent in bark or stem as compared to leaves. The content of BA was found to be the highest, 0.43%, in Dillenia indica bark.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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