JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 27: Issue 1
New Report of Triterpenoid Betulinic Acid along with Oleanolic Acid from Achyranthes aspera by Reversed-Phase-Ultra Flow Liquid Chromatographic Analysis and Confirmation Using High-Performance Thin-Layer Chromatographic and Fourier Transform-Infrared Spectroscopic Techniques
Authors:
Sandeep Pai Indian Council of Medical Research (ICMR), Nehru Nagar Regional Medical Research Centre (RMRC) Belgaum, Karnataka 590 010 India

Search for other papers by Sandeep Pai in
Current site
Google Scholar
PubMed Close
,
Vinayak Upadhya Indian Council of Medical Research (ICMR), Nehru Nagar Regional Medical Research Centre (RMRC) Belgaum, Karnataka 590 010 India

Search for other papers by Vinayak Upadhya in
Current site
Google Scholar
PubMed Close
,
Harsha Hegde Indian Council of Medical Research (ICMR), Nehru Nagar Regional Medical Research Centre (RMRC) Belgaum, Karnataka 590 010 India

Search for other papers by Harsha Hegde in
Current site
Google Scholar
PubMed Close
,
Rajesh Joshi Indian Council of Medical Research (ICMR), Nehru Nagar Regional Medical Research Centre (RMRC) Belgaum, Karnataka 590 010 India

Search for other papers by Rajesh Joshi in
Current site
Google Scholar
PubMed Close
, and
Sanjiva Kholkute Indian Council of Medical Research (ICMR), Nehru Nagar Regional Medical Research Centre (RMRC) Belgaum, Karnataka 590 010 India

Search for other papers by Sanjiva Kholkute in
Current site
Google Scholar
PubMed Close
Pages:
38–41
Online Publication Date:
06 Feb 2014
Publication Date:
01 Feb 2014
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.27.2014.1.7
Keywords:
RP-UFLC; HPTLC; FT-IR; Betulinic acid; Oleanolic acid
Restricted access

Triterpenoid betulinic acid (BA) was detected, quantified, and reported for the first time from leaf extract of Achyranthes aspera along with much known oleanolic acid (OA). Extraction was achieved using ultrasonic exposure, and reversed-phase.ultra flow liquid chromatographic (RP.UFLC) technique was employed during investigation. RP.UFLC separation was achieved on a Hibar 250–4.6 mm, 5 μ, Lichrospher 100, C18e column using methanol and water (90:10) as mobile phase with pH adjusted to 5.0 using glacial acetic acid (GAA) in an isocratic mode. The content of BA (0.25 mg g−1 fresh weight [FW]) was ∼75% higher than OA (0.06 mg g−1 FW). These results suggest BA to be the major triterpenoid compared to OA in the leaf of A. aspera. High-performance thin-layer chromatography (HPTLC) separation of the two triterpenic acids (oleanolic and betulinic acid) was also achieved on silica gel G 60 F254, 50 ×~ 100 mm glass TLC plates, using benzene, ethyl acetate, and formic acid as solvent system in a ratio of 67.9:22.7:9.4. Anisaldehyde reagent was used for detection. The method was used for the screening of oleanolic and betulinic acids from A. aspera leaf extract. Similarly, Fourier transform-infrared (FT-IR) spectroscopic analysis was done in the mid IR region of 400–4000 cm−1 with 64 scan speed using OMNIC 8.1 (ver. 8.1.210) software. The results were also supported by HPTLC and FT-IR data.

  • V. Upadhya, D. Mesta, H.V. Hegde, S. Bhat, S.D. Kholkute, J. Econ. Taxon. Bot. 33 (2009) 300–308.

    Kholkute SD , '' (2009 ) 33 J. Econ. Taxon. Bot. : 300 -308 .

  • A. Aziz, M. Rahman, A.K. Mondal, T. Muslim, A. Rahman, A. Quader, Pharmaceutical J. 4 (2005) 1816–1820.

    Quader A , '' (2005 ) 4 Pharmaceutical J. : 1816 -1820 .

  • A. Bagavan, A.A. Rahuman, C. Kamaraj, K. Geetha, Parasitol. Res. 103 (2008) 223–229.

    Geetha K , '' (2008 ) 103 Parasitol. Res. : 223 -229 .

  • P.R. Subbarayana, M. Sarkarb, S. Impellizzeric, F. Raymoc, B.L. Lokeshward, P. Kumare, R.P. Agarwala, B. Ardalana, J. Ethnopharmacol. 131 (2010) 78–82.

    Ardalana B , '' (2010 ) 131 J. Ethnopharmacol. : 78 -82 .

  • A. Chakraborty, A. Brantner, T. Mukainaka, Y. Nobukuni, M. Kuchide, T. Konoshima, H. Tokuda, H. Nishino, Cancer Lett. 177 (2002) 1–5.

    Nishino H , '' (2002 ) 177 Cancer Lett. : 1 -5 .

  • T. Vetrichelvan, M. Jegadeesan, Phytother. Res. 17 (2003) 77–79.

    Jegadeesan M , '' (2003 ) 17 Phytother. Res. : 77 -79 .

  • C.C. Barua, A. Talukdar, S.A. Begum, B. Buragohain, J.D. Roy, R.S. Borah, M. Lahkar, Pharmacologyonline 2 (2009) 587–594.

    Lahkar M , '' (2009 ) 2 Pharmacologyonline : 587 -594 .

  • C.C. Barua, S.A. Begum, A. Talukdar, P. Borah, Pharmacologyonline 1 (2010a) 183–191.

    Borah P , '' (2010 ) 1 Pharmacologyonline : 183 -191 .

  • C.C. Barua, A. Talukdar, S.A. Begum, L.C. Lahon, D.K. Sarma, D.C. Pathak, P. Borah, Indian J. Exp. Biol. 48 (2010b) 817–821.

    Borah P , '' (2010 ) 48 Indian J. Exp. Biol. : 817 -821 .

  • K. Sandhyakumar, R.G. Boby, M. Indira, Indian J. Exp. Biol. 40 (2002) 1307–1309.

    Indira M , '' (2002 ) 40 Indian J. Exp. Biol. : 1307 -1309 .

  • H. Mukherjee, D. Ojha, P. Bag, H.S. Chandel, S. Bhattacharyya, T.K. Chatterjee, P.K. Mukherjee, S. Chakraborti, D. Chattopadhyay, Microbiol. Res. 168 (2013) 238–244.

    Chattopadhyay D , '' (2013 ) 168 Microbiol. Res. : 238 -244 .

  • N. Suh, T. Honda, H.J. Finlay, A. Barchowsky, C. Williams, N.E. Benoit, Q.-W. Xie, C.N. Gordon, W. Gribble, M.B. Spon, Cancer Res. 58 (1998) 717–723.

    Spon MB , '' (1998 ) 58 Cancer Res. : 717 -723 .

  • S.R. Pai, M.S. Nimbalkar, N.V. Pawar, G.B. Dixit, Ind. Crops Prod. 34 (2011) 1458–1464.

    Dixit GB , '' (2011 ) 34 Ind. Crops Prod. : 1458 -1464 .

  • T. Galgon, D. Hoke, B. Drager, Phytochem. Anal. 10 (1999) 187–190.

    Drager B , '' (1999 ) 10 Phytochem. Anal. : 187 -190 .

  • M.G. Moghaddam, F.B.H. Ahmad, A. Samzadeh-Kermani, Pharmacol. Pharm. 3 (2012) 119–123.

    Samzadeh-Kermani A , '' (2012 ) 3 Pharmacol. Pharm. : 119 -123 .

  • S. Srivastava, M.M. Gupta, R.K. Verma, S.C. Singh, S. Kumar, J. Indian Chem. Soc. 79 (2002) 286–288.

    Kumar S , '' (2002 ) 79 J. Indian Chem. Soc. : 286 -288 .

  • M.S. Nimbalkar, S.R. Pai, N.V. Pawar, D. Oulkar, G.B. Dixit, Food Chem. 134 (2012) 2565–2569.

    Dixit GB , '' (2012 ) 134 Food Chem. : 2565 -2569 .

  • N. Pawar, S. Pai, M. Nimbalkar, G. Dixit, Food Chem. 126 (2011) 1330–1336.

    Dixit G , '' (2011 ) 126 Food Chem. : 1330 -1336 .

  • K. Naumoska, B. Simonovska, A. Albreht, I. Vovk, J. Planar Chromatogr. 26 (2013) 125–131.

    Vovk I , '' (2013 ) 26 J. Planar Chromatogr. : 125 -131 .

  • G. Bringmann, W. Saeb, L. Ake Assi, G. Francois, A.S. Sankaranarayanan, K. Peters, E.M. Peters, Planta Med. 63 (1997) 255–257.

    Peters EM , '' (1997 ) 63 Planta Med. : 255 -257 .

  • K.H. Bae, S.M. Lee, E.S. Lee, J.S. Lee, J.S. Kang, Yakhak Hoechi 40 (1996) 558–562.

    Kang JS , '' (1996 ) 40 Yakhak Hoechi : 558 -562 .

  • B.H. de Oliveira, C.A. Santos, A.P. Espíndola, Phytochem. Anal. 13 (2002) 95–98.

    Espíndola AP , '' (2002 ) 13 Phytochem. Anal. : 95 -98 .

  • U.D. Neue, HPLC Columns Theory, Technology, and Practice, Wiley-VCH, New York, 1997.

    Neue UD , '', in HPLC Columns Theory, Technology, and Practice , (1997 ) -.

  • E.E. Kovač-Bešović, K. Durić, Z. Kalođera, E. Sofić, Boston J. Basic Med. Sci. 9 (2009) 31–38.

    Sofić E , '' (2009 ) 9 Boston J. Basic Med. Sci. : 31 -38 .

  • S. Cînta-Pînzaru, N. Leopold, W. Kiefer, Talanta 57 (2002) 625–631.

    Kiefer W , '' (2002 ) 57 Talanta : 625 -631 .
  • Collapse
  • Expand
Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2024 11 0 0
Jul 2024 5 0 0
Aug 2024 15 0 0
Sep 2024 8 0 0
Oct 2024 10 0 0
Nov 2024 30 0 0
Dec 2024 34 0 0