JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 27: Issue 2
Novel Thin-Layer Chromatographic—Densitometric Method for the Quantification of Betulinic Acid in Nagod (Vitex negundo L.}) and Its Herbal Formulations
Authors:
Rajendra Patel K. B. Institute of Pharmaceutical Education and Research Department of Pharmaceutical Analysis Gandhinagar Gujarat India

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Jigar Patel K. B. Institute of Pharmaceutical Education and Research Department of Pharmaceutical Analysis Gandhinagar Gujarat India

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Priti Trivedi K. B. Institute of Pharmaceutical Education and Research Department of Pharmaceutical Analysis Gandhinagar Gujarat India

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Pages:
102–106
Online Publication Date:
14 Mar 2014
Publication Date:
01 Apr 2014
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.27.2014.2.6
Keywords:
Betulinic acid; HPTLC
Restricted access

A selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the analysis of Nagod samples and its formulations for their betulinic acid content. The method involves densitometric evaluation of betulinic acid after resolving it by HPTLC on silica gel plates with toluene—acetone—formic acid (17:3:0.2, v/v/v) as the mobile phase. For densitometric evaluation, peak areas were recorded at 445 nm after derivatizing the resolved bands with anisaldehyde—sulphuric acid reagent. The relation between the concentration of betulinic acid and the corresponding peak areas was found to be linear within the range of 200–1200 ng/spot. The method was validated for linearity range, precision, limit of detection (LOD), limit of quantification (LOQ), and accuracy and was demonstrated for quantification of betulinic acid in Vitex negundo L. plant parts and its commercial polyherbal formulations. The method was able to identify and quantify betulinic acid in complex mixtures of phytochemicals and could be extended to marker-based standardization of plant samples, extracts, and its polyherbal formulations.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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