JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 27: Issue 4
Simultaneous estimation of kaempferol, rutin, and quercetin in various plant products and different dosage forms of Bhuiamla and Amla
Authors:
Supriya Jirge S.N.D.T. Women’s University C.U. Shah College of Pharmacy Mumbai 400 049 India

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,
Pratima Tatke S.N.D.T. Women’s University C.U. Shah College of Pharmacy Mumbai 400 049 India

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, and
Satish Gabhe S.N.D.T. Women’s University C.U. Shah College of Pharmacy Mumbai 400 049 India

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Pages:
267–273
Online Publication Date:
30 Jul 2014
Publication Date:
01 Aug 2014
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.27.2014.4.6
Keywords:
Kaempferol; Rutin; Quercetin; High-performance thin-layer chromatography
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Standardization has become mandatory for global acceptance of herbal oriental medicines which lack validated methods of analysis due to their complex nature. Thus, a simple and fast method was developed for the simultaneous quantitative determination of three biologically active flavonoid compounds, i.e., kaempferol, rutin, and quercetin in extracts and marketed formulations containing Phyllanthus niruri (Bhuiamla) and Emblica officinalis (Amla). The separation was performed on thin-layer chromatography (TLC) aluminum plates precoated with silica gel 60 F254. Good separation was achieved in the mobile phase composed of chloroform-methanol-formic acid and detected at wavelength 254 nm in reflectance-absorbance mode. The developed method was well validated by different parameters mentioned in International Conference on Harmonization (ICH) guidelines such as linearity, accuracy, precision, etc. The linear regression data for the calibration plots showed a good linear relationship with r = 0.9997, 0.9998, and 0.9998 for kaempferol, rutin, and quercetin, respectively. The validated method was successfully applied to determine the contents of three biomarkers in a single run in Bhuiamla Vati and capsule (Ayurvedic and modern solid dosage form, respectively) and Amla Vati and Amla tincture (homeopathic preparation). The present high-performance thin-layer chromatographic (HPTLC) method, which was used for the standardization of various marketed traditional medicines of two different medicinal plants, is being reported the first time with these three biomarkers.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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