JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 27: Issue 5
Simultaneous quantitation of piperine and piperlongumine in the fruit of Piper longum Linn. by validated high-performance thin-layer chromatography-densitometric method
Authors:
Kilambi Pundarikakshudu L. J. Institute of Pharmacy, Between Sarkhej Circle and Kataria Motors Department of Pharmacognosy S. G. Road Ahmedabad 382 210 India

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Anilkumar Sharma Parul Institute of Pharmacy, Limda 391 760 Waghodia Taluk, Vadodara District Gujarat India

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Chaitanya Bhatt K.B. Institute of Pharmaceutical Education and Research Sector - 23, GH - 6, Gandhinagar 382 023 Gujarat India

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Niranjan Kanaki K.B. Institute of Pharmaceutical Education and Research Sector - 23, GH - 6, Gandhinagar 382 023 Gujarat India

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Pages:
362–366
Online Publication Date:
08 Oct 2014
Publication Date:
01 Oct 2014
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.27.2014.5.6
Keywords:
High-performance thin-layer chromatography; Densitometry; Piperine; Piperlongumine
Restricted access

The fruit of Piper longum Linn. (family: Piperaceae), known as pippali in India, is a reputed drug of Ayurveda (the Indian system of medicine). It contains many amides of piperic acid among which piperine and piperlongumine are important from biological point of view. In the present work, we quantified these two marker compounds from the fruits of P. longum by high-performance thin-layer chromatography (HPTLC) with densitometry. The method was found to be precise. Relative standard deviation (RSD) values for intra-day analyses were in the range of 1.05 to1.07% (piperine) and 1.12 to 1.16% (piperlongumine) and, for inter-day analyses, the values were in the range of 1.11 to 1.21% (piperine) and 1.02 to1.18% (piperlongumine). Instrumental RSD values were 0.64 and 0.78% for piperine and piperlongumine, respectively. Accuracy of the method was evaluated by carrying a recovery study at three different levels for the two compounds. Average recoveries were found to be 99.23% for piperine and 99.26% for piperlongumine. P. longum fruits obtained from Bombay market showed 0.213 ± 0.009% w/w piperine and 0.364 ± 0.014% w/w piperlongumine when analyzed by the proposed method. Similarly, sample fruits obtained from Ahmedabad market had 0.85 ± 0.026% piperine and 2.70 ± 0.065% of piperlongumine. This TLC-densitometric method was found to be simple, precise, specific, sensitive, and accurate. So it can be used in routine quality control for the simultaneous analysis of piperine and piperlongumine from P. longum fruits and piperine alone from Piper nigrum fruits.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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