JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 27: Issue 6
Multiple marker-based evaluation of Kalanchoe pinnata, Bombax ceiba, and Morus alba leaves: Quantification of α-amyrin, lupeol, and β-sitosterol using high-performance thin-layer chromatography
Authors:
Hemendra Yadav National Institute of Pharmaceutical Education and Research — Ahmedabad Department of Natural Products S. G. Highway Thaltej, Ahmedabad 380054 Gujarat India

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Mukesh Kumar National Institute of Pharmaceutical Education and Research — Ahmedabad Department of Natural Products S. G. Highway Thaltej, Ahmedabad 380054 Gujarat India

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Manish Nivsarkar B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre Department of Pharmacology and Toxicology S. G. Highway Thaltej, Ahmedabad 380054 Gujarat India

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Sheetal Anandjiwala National Institute of Pharmaceutical Education and Research — Ahmedabad Department of Natural Products S. G. Highway Thaltej, Ahmedabad 380054 Gujarat India

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Pages:
438–443
Online Publication Date:
17 Nov 2014
Publication Date:
01 Dec 2014
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.27.2014.6.6
Keywords:
Kalanchoe pinnata ; Bombax ceiba ; Morus alba ; High-performance thin-layer chromatography; α-Amyrin; Lupeol; β-Sitosterol
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Kalanchoe pinnata (Crassulaceae), Bombax ceiba (Bombaceae), and Morus alba (Moraceae) are important drugs in Indian and Chinese Systems of Medicine. Leaves of K. pinnata are reported to show anti-inflammatory, antifungal, and antibacterial activity and are reported to contain quercetin, kaempferol, α-amyrin, β-sitosterol, ferulic, and caffeic acid. Leaves of B. ceiba are reported for the treatment of diarrhea, dysentery, gonorrhea, and bladder ulceration and are reported to contain shamimin, lupeol, β-sitosterol, and α-amyrin. Leaves of M. alba are reported to show anti-inflammatory, emollient, hypolipidemic, and diuretic activity and are reported to contain quercetin, rutin, moracetin, and artocarpin. In the present work, a method for simultaneous quantification of three marker compounds, viz., α-amyrin, lupeol, and β-sitosterol from the leaves of K. pinnata, B. ceiba, and M. alba was developed and validated as per International Conference on Harmonization (ICH) guidelines. This is the first report of simultaneous quantification of these three compounds. α-Amyrin, lupeol, and β-sitosterol resolved at RF 0.71, 0.28, and 0.16, respectively, on thin-layer chromatography (TLC) when developed in the solvent system of toluene-ethyl acetate (9.5:0.5). The linearity range for α-amyrin, lupeol, and β-sitosterol was found to be 160–560, 150–900, and 80–480 ng spot−1, respectively, with correlation coefficients (r values) of 0.999, 0.997, and 0.995, respectively. The amount of α-amyrin, lupeol, and β-sitosterol were found to be in range of 0.013–0.047, 0.046–0.318, and 0.028–0.123 % w/w, respectively. The developed methods were found to be accurate, precise, and reproducible and can be used for routine quality control of herbal material and formulations containing K. pinnata, B. ceiba, and M. alba.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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