JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 28: Issue 1
Development of a densitometric high-performance thin-layer chromatographic method for the quantitative analysis of biomarker lupeol in the leaves of different species of genus Ficus
Authors:
Perwez Alam King Saud University Department of Pharmacognosy, College of Pharmacy Riyadh Kingdom of Saudi Arabia

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Omer Basudan King Saud University Department of Pharmacognosy, College of Pharmacy Riyadh Kingdom of Saudi Arabia

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Nasir Siddiqui King Saud University Department of Pharmacognosy, College of Pharmacy Riyadh Kingdom of Saudi Arabia

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Adnan Al-Rehaily King Saud University Department of Pharmacognosy, College of Pharmacy Riyadh Kingdom of Saudi Arabia

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Saleh Alqasoumi King Saud University Department of Pharmacognosy, College of Pharmacy Riyadh Kingdom of Saudi Arabia

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Maged Abdel-Kader Salman Bin Abdulaziz University Department of Pharmacognosy, College of Pharmacy Al-Kharj Kingdom of Saudi Arabia

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Abd Donia Salman Bin Abdulaziz University Department of Pharmacognosy, College of Pharmacy Al-Kharj Kingdom of Saudi Arabia

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Prawez Alam Salman Bin Abdulaziz University Department of Pharmacognosy, College of Pharmacy Al-Kharj Kingdom of Saudi Arabia

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Pages:
30–35
Online Publication Date:
27 Jan 2015
Publication Date:
01 Feb 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.28.2015.1.5
Keywords:
Quality control; Lupeol; High-performance thin-layer chromatography; Validation
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A simple and sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the evaluation of biomarker lupeol in the leaves of five different species of genus Ficus (Ficus carica, Ficus nitida, Ficus ingens, Ficus palmata, and Ficus vest) belonging to family Moraceae. Chromatography was performed on glass-backed silica gel 60 F254 precoated HPTLC plates with solvents toluene-methanol (9:1, v/v) as the mobile phase. After development, the HPTLC plate was derivatized with p-anisaldehyde reagent, scanned, and quantified at 540 nm. The system was found to give compact spot for lupeol at RF = 0.32 ± 0.01. The precisions and accuracy (n = 6) for lupeol were found to be 1.47–1.64% and 1.63–1.86%, and 99–100% and 99.4–99.7%, respectively, for inter-day and intra-day. Lupeol was found to be present in four species, i.e., F. carica (0.4%, w/w), F. nitida (1.4%, w/w), F. palmata (0.33%, w/w), and F. vest (0.59%, w/w), while it was absent in F. ingens. The statistical analysis proved that the developed method is reproducible and selective. The developed method can be used as an important tool to assure the therapeutic dose of active ingredients in herbal formulations as well as for standardization and quality control of bulk drugs and in-process formulations. This method can also be employed for the further study of degradation kinetics and determination of lupeol in plasma and other biological fluids.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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