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Saurabh Satija Maharshi Dayanand University, Rohtak, Haryana — 124 001, India

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Swati Malik Maharshi Dayanand University, Rohtak, Haryana — 124 001, India

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Munish Garg Maharshi Dayanand University, Rohtak, Haryana — 124 001, India

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A straightforward, delicate, and fast elite thin-layer chromatographic system (high-performance thin-layer chromatography [HPTLC]) has been produced and validated for the quantitative determination of berberine in Tinospora cordifolia. The chromatographic development was completed on HPTLC plates precoated with silica gel 60 F254 utilizing a blend of methanol, acetic acid, and water (8:1:1, v/v) as the mobile phase. Detection was completed densitometrically at 366 nm. The RF estimation of berberine was observed to be 0.71 ± 0.02. The system was validated according to the International Conference on Harmonization (ICH) guidelines regarding linearity, precision, accuracy, robustness, and so forth. The calibration curve was observed to be straight over a scope of 120–360 ng spot−1 with a regression coefficient of 0.971. The accuracy was observed to be as high as 98.56%, and the relative standard deviation (% RSD) values for intra-day and between-day variations were under 2%. The system showed high affectability and specificity. The strategy is new, basic, and economical for the routine estimation of berberine in T. cordifolia plant tests to help the commercial ventures and, in addition, scientists for their quick touchy determination of critical phytoconstituent berberine, requiring little to no effort in routine examination.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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