JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 29: Issue 5
Chemical fingerprinting and quantification of biomarker wedelolactone in different sources of “Bhringaraja” by high-performance thin-layer chromatography and method validation
Authors:
Kirankumar Hullatti KLE University, Nehru Nagar, Belagavi, Karnataka 590 010, India

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Analee Rodrigues KLE University, Nehru Nagar, Belagavi, Karnataka 590 010, India

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Vinod Mannur KLE University, Nehru Nagar, Belagavi, Karnataka 590 010, India

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Vinayak Mastiholimath KLE University, Nehru Nagar, Belagavi, Karnataka 590 010, India

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Vijay Kumar Rasal KLE University, Nehru Nagar, Belagavi, Karnataka 590 010, India

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Pages:
330–335
Online Publication Date:
Oct 2016
Publication Date:
01 Oct 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2016.29.5.1
Keywords:
Eclipta alba ; Quality-control parameters; Wedelia calendulacea ; Wedelia trilobata
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Two different botanical sources, Eclipta alba and Wedelia calendulacea are used as “Bhringaraja” in the Ayurvedic s ystem o f medicine. In the present study, an effort has been made to evaluate different sources by using high-performance thin-layer chromatography (HPTLC) as an analytical tool. Wedelolactone, one of the primary constituents of these plants, was taken as the marker compound for the evaluation. An HPTLC method was developed and validated for the evaluation of different sources of “Bhringaraja”. The chromatographic system was developed using silica gel 60 F254 HPTLC plates with the mobile phase toluene–chloroform–ethyl alcohol–formic acid (5:4:1:0.5, v/v). Linearity was found between the concentration ranges of 80 to 280 ng spot−1 (R2: 0.9994), and limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.36 ng spot−1 and 1.09 ng spot−1. The study has shown the presence of wedelolactone at the concentration of 0.26% w/w and 0.05% w/w in E. alba and W. calendulacea, respectively, whereas it was absent in another closely related species, Wedelia trilobata. At the same time, all the three plants were subjected to evaluation of quality-control parameters as per the World Health Organization (WHO) guidelines. Certain parameters such as foaming index, total alkaloids, and total bitter principles were significantly different in the three plants. Hence, the present HPTLC method development, and the validation and evaluation of quality-control parameters would be helpful in the standardization of individual plants and their formulations.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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