JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 29: Issue 6
Selection of crude Solidago virgaurea L. ssp. extracts based on two fast and efficient chromatographic methods (high-performance thin-layer chromatography or high-performance liquid chromatography) for the quantification of total saponins
Authors:
Lise Laurençon CNRS — Université Nice Sophia Antipolis, Parc Valrose, 06108 Nice CEDEX 2, France

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Thi Kieu Tien Do CNRS — Université Nice Sophia Antipolis, Parc Valrose, 06108 Nice CEDEX 2, France
BotaniCert, Espace Jacques-Louis Lions, 4 traverse Dupont, 06130 Grasse, France

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Francis Hadji-Minaglou BotaniCert, Espace Jacques-Louis Lions, 4 traverse Dupont, 06130 Grasse, France

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Sylvain Antoniotti CNRS — Université Nice Sophia Antipolis, Parc Valrose, 06108 Nice CEDEX 2, France

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Xavier Fernandez CNRS — Université Nice Sophia Antipolis, Parc Valrose, 06108 Nice CEDEX 2, France

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Pages:
410–416
Online Publication Date:
Dec 2016
Publication Date:
01 Dec 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2016.29.6.2
Keywords:
High-performance liquid chromatography—evaporative light scattering detection; High-performance thin-layer chromatography; Densitometry; In-situ hydrolysis; Bioactive natural extracts
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A previous study on Solidago virgaurea extracts showed an inhibiting activity of Candida albicans yeast—hyphal transition due to a mixture of triterpene saponins, leading to applications in the field of oral care products. Such applications require the development of an efficient, fast, and simple quantification method of S. virgaurea total saponins. Two methods were developed: the first was based on high-performance liquid chromatography (HPLC) separation with gradient elution and evaporative light scattering detection (ELSD); the second was based on high-performance thin-layer chromatography (HPTLC) separation with in-situ hydrolysis followed by densitometric measurements at 366 nm. Both calibration curves showed good linear regressions (R2 > 0.99) within the range of the concentrations tested. The limit of detection (LOD) and the limit of quantitation (LOQ) were 2–4 μg and 10–16 μg, respectively. The intra- and inter-day variations were studied and found to remain below 5.6% in terms of relative standard deviation (RSD). The recoveries were 97.02–101.91% with RSD of 0.3–1.96% for spiked sample. Both validated methods were successfully applied to the analysis of total saponins in different S. virgaurea samples. In particular, a harvesting study could be supported by these methods to identify the most relevant vegetal parts to extract, and the locations and vintage to collect, to obtain the desired active saponins. HPLC is the recommended method for precise analyses, but HPTLC is the most efficient for the fast analysis of multiple samples.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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