JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 30: Issue 4
Development and validation of a high-performance thin-layer chromatography method for the determination of artesunate and amodiaquine in tablet formulations
Authors:
Yonah H. Mwalwisi Tanzania Food and Drug Authority, Dar es Salaam, Tanzania
University of Würzburg, Am Hubland, 97074 Würzburg, Germany

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Seraphina C. Omolo Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

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Ludwig Hoellein University of Würzburg, Am Hubland, 97074 Würzburg, Germany

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Danstan H. Shewiyo Tanzania Food and Drug Authority, Dar es Salaam, Tanzania

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Ulrike Holzgrabe Tanzania Food and Drug Authority, Dar es Salaam, Tanzania

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Eliangiringa Kaale Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

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Pages:
307–312
Online Publication Date:
Aug 2017
Publication Date:
01 Aug 2017
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2017.30.4.11
Keywords:
Artesunate; Amodiaquine; Counterfeit and substandard medicines; Planar chromatography; Densitometry; Quality control
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A simple, cost-effective, precise, accurate, and rapid planar chromatographic method was developed and validated for the separation and determination of amodiaquine and artesunate in tablet formulations. Both compounds were determined using high-performance thin-layer chromatography plates and a mobile phase composed of toluene, acetonitrile, methanol, ammonium acetate, and triethylamine in the ratio 10:5:3:1:0.5 (% v/v). Amodiaquine was evaluated densitometrically at a detection wavelength of λ = 345 nm, whereas artesunate was determined fluorimetrically at λ = 503 nm. The method was linear in the concentration ranges of 0.093–0.280 μg spot−1 for artesunate and 0.250–1.250 μg spot−1 for amodiaquine, respectively.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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