JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 31: Issue 4
Box–Behnken design-assisted optimization for simultaneous estimation of quercetin, kaempferol, and keto-β-boswellic acid by high-performance thin-layer chromatography method
Authors:
Karuna Gohel Anand Pharmacy College, Anand, India

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,
Kalpana Patel Anand Pharmacy College, Anand, India

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,
Purvi Shah Anand Pharmacy College, Anand, India

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,
Lal Hingorani Pharmanza Herbals Pvt. Ltd., Dharmaj, India

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, and
Tejal Gandhi Anand Pharmacy College, Anand, India

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Pages:
318–326
Online Publication Date:
Aug 2018
Publication Date:
01 Aug 2018
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2018.31.4.7
Keywords:
High-performance thin-layer chromatography; Quercetin; Kaempferol; Keto-β-boswellic acid; Box–Behnken design; Validation
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A simple, specific, and quantitative high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous determination of quercetin, kaempferol, and keto-β-boswellic acid in the herbal extracts of Spinacia oleracea and Boswellia serrata. Chromatographic development was performed using n-hexane‒ethyl acetate‒glacial acetic acid (6.4:3.6:0.2, % v/v) as optimized mobile phase on TLC aluminum plate precoated with silica gel 60 F254 and optimized resolution between quercetin and kaempferol (R12) and between kaempferol and KBBA (R23) were 2.11 and 2, respectively. The detection of quercetin, kaempferol, and keto-β-boswellic acid was carried out at 272 nm. Box‒Behnken design was applied for optimization of the chromatographic conditions and combination of factors such as the volume of n-hexane (A), solvent front (B), and chamber saturation time (C) which were likely to affect RF, and resolution of quercetin, kaempferol, and keto-β-boswellic acid were identified from preliminary trials and further optimized using response surface design. The drugs were satisfactorily resolved with RF values of 0.22 ± 0.02, 0.40 ± 0.02, and 0.54 ± 0.02 for quercetin, kaempferol, and keto-β-boswellic acid, respectively. The linear ranges were found to be 1500–3500, 200–600, and 1040–3120 ng band−1 for quercetin, kaempferol, and keto-β-boswellic acid. The accuracy and precision measured were less than 2% relative standard deviation for all markers. The sensitivity of the method in terms of limit of detection (LOD) and limit of quantitation (LOQ) were measured. Among three factors, the significant factor found was the volume of hexane that resulted in a change in the RF of all three markers. The proposed method was found to be accurate, precise, reproducible, robust, and specific and can be applicable for the simultaneous determination of quercetin, kaempferol, and keto-β-boswellic acid for the quality control testing of extracts and medicinal plants.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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