Experimental evaluation of solvent elution strength in reversed-phase thin-layer chromatography on RP2 and RP8 plates

Łukasz Komsta; Robert Skibiński; Sebastian Gadowski; Joanna Wróbel; Karolina Skrzypiec

doi:10.1556/1006.2018.31.5.1

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JPC - Journal of Planar Chromatography - Modern TLC

Volume/Issue:: Volume 31: Issue 5

Experimental evaluation of solvent elution strength in reversed-phase thin-layer chromatography on RP2 and RP8 plates

Authors:

Łukasz Komsta

Łukasz Komsta Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland

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Robert Skibiński

Robert Skibiński Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland

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Sebastian Gadowski

Sebastian Gadowski Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland

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Joanna Wróbel

Joanna Wróbel Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland

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, and

Karolina Skrzypiec

Karolina Skrzypiec Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland

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Pages:: 351–354

Online Publication Date:: Oct 2018

Publication Date:: 01 Oct 2018

Article Category:: Research Article

DOI:: https://doi.org/10.1556/1006.2018.31.5.1

Keywords:: RP8; RP2; Solvent strength; Experimental design

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The eluotropic strength on RP2 and RP8 plates was investigated for 7 chromatographic grade solvents miscible with water as a diluent (acetone, acetonitrile, dioxane, ethanol, isopropanol, methanol, and tetrahydrofuran) as well as for water itself. Thirty-five model compounds were used as a test set in the investigation. The use of a modern chemometric mixture design concept allowed separate estimation of each solvent effect from the other results with a relatively small error (about 0.05 of R_M value for both adsorbents). Principal component analysis was used as an additional tool and revealed that only one parameter (mean eluotropic strength) is satisfactory to explain the overall variability in the retention datasets. The bootstrapping approach was also used to check the distribution and uncertainty of the obtained values. It can be concluded that all solvents have quite similar elution strength for both adsorbents. The obtained values can be used as a reference during the optimization of thin-layer chromatography (TLC) systems.

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JPC - Journal of Planar Chromatography - Modern TLC

Print ISSN:: 0933-4173

Online ISSN:: 1789-0993

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Journal of Planar Chromatography - Modern TLC
Language	English
Size	A4
Year of Foundation	1988
Volumes per Year	1
Issues per Year	6
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0933-4173 (Print)
ISSN	1789-0993 (Online)