JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 32: Issue 4
Simultaneous High-Performance Thin-Layer Chromatographic Quantification of Withaferin A and Withanolide A in Solanum nigrum L. “Black Nightshade”
Authors:
Raman Preet Punjabi University, Patiala, India

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Raghbir Chand Gupta Punjabi University, Patiala, India

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Manjot Kaur Punjabi University, Patiala, India

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Pages:
339–342
Online Publication Date:
Aug 2019
Publication Date:
01 Aug 2019
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2019.32.4.10
Keywords:
Solanum nigrum ; High-performance thin-layer chromatography; Withanolide A; Withaferin A
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Summary

A simple, selective, sensitive, and precise high-performance thin-layer chromatographic technique was followed to determine the diversity within accessions of Solanum nigrum. Fruit, stem, leaf, and root samples extracted in methanol were used to quantify withanolide A and withaferin A. Significant difference has been detected among the different plant parts. The maximum amount of withaferin A has been found to be present in the leaf sample (71.65 ± 2.86 mg g−1 DW), followed by the stem sample, while the least amount of withaferin A has been detected in the mature fruit sample (5.97 ± 1.91 mg g−1 DW).

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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