Authors:
G. WeselohHahn-Meitner-Institut Berlin Glienicker Straße 100 D-14109 Berlin Germany

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M. KühbacherHahn-Meitner-Institut Berlin Glienicker Straße 100 D-14109 Berlin Germany

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H. BertelsmannHahn-Meitner-Institut Berlin Glienicker Straße 100 D-14109 Berlin Germany

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M. ÖzaslanHahn-Meitner-Institut Berlin Glienicker Straße 100 D-14109 Berlin Germany

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A. KyriakopoulosHahn-Meitner-Institut Berlin Glienicker Straße 100 D-14109 Berlin Germany

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A. KnöchelUniversität Hamburg Institut für Anorganische Chemie Martin-Luther-King Platz 6 D-20146 Hamburg Germany

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Abstract  

For the analysis of metal-containing proteins, sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been combined with synchrotron radiation X-ray fluorescence (SRXRF). In a pilot study the applicability of this combined method was tested in the analysis of metalloaded apoazurin and of the selenoproteins in rat testis homogenate. It was shown that it can be applied in the determination of the major stable binding forms of trace elements. After further improvement of the limits of detection the method will allow the analysis of trace element-containing proteins present in the samples at low concentrations.

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
1
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)