View More View Less
  • 1 Sohag University, 82524 Sohag, Egypt
Restricted access

Salinity is one of the major abiotic stress factors affecting series of morphological, physiological, metabolic and molecular changes in plant growth. The effect of different concentrations (0, 25, 50, 100 and 150 mM) of NaCl on the vegetative growth and some physiological parameters of karkade (Hibiscus sabdariffa var. sabdariffa) seedling were investigated. NaCl affected the germination rate, delayed emergence and retarded vegetative growth of seedlings. The length of seedling as well as the leaf area was significantly reduced. The fresh weight remained lower in NaCl treated seedlings compared to control. NaCl at 100 and 150 mM concentrations had significant effect on the dry matter contents of the treated seedlings. The chloroplast pigments in the treated seedlings were affected, suggesting that the NaCl had a significant effect on the chlorophyll and carotenoid biosynthesis. The results showed that the salt treatments induced an increase in proline concentration of the seedlings. The osmotic potential (ψs) of NaCl treated seedlings decreased with increasing NaCl concentrations. Salt treatments resulted in dramatic quantitative reduction in the total sterol percent compared with control ones. Salt stress resulted in increase and decrease of Na+ and K+ ions, respectively. NaCl salinity increased lipid peroxidation. SDS-PAGE was used to evaluate protein pattern after applying salt stress. High molecular weight proteins were intensified, while low molecular weight proteins were faint. NaCl at 100 and 150 mM concentration distinguished with new protein bands. Salt stress induced a new peroxidase bands and increased the band intensity, indicating the protective role of peroxidase enzyme.

  • 1.

    A.O.A.C. (1986) Official analysis of the association of official analytical chemist. 14th ed. Washington, D.C.

  • 2.

    Akinci, I. E., Akinci, S., Yilmaz, K., Dikici, H. (2004) Response of eggplant varieties (Solanum melongena) to salinity in germination and seedling stages. New Zealand J. Crop Horticul. Sci. 32, 193200.

    • Search Google Scholar
    • Export Citation
  • 3.

    Ali, H. M., Siddiqui, M. H., Basalah, M. O., Al-Whaibi, M. H., Sakran, A. M., Al-Amri, A. (2012) Effects of gibberellic acid on growth and photosynthetic pigments of Hibiscus sabdariffa L. under salt stress. Afr. J. Biotechnol. 11, 800804.

    • Search Google Scholar
    • Export Citation
  • 4.

    Allakhverdiev, S. I., Sakamoto, A., Nishiyama, Y., Inaba, M., Murata, N. (2000) Photosystems I and II in Synechococcus sp. Plant Physiol. 123, 10471056.

    • Search Google Scholar
    • Export Citation
  • 5.

    Almansouri, M., Kine, T. J. M., Lutts, S. (2001) Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant Soil 231, 243254.

    • Search Google Scholar
    • Export Citation
  • 6.

    Andriolo, J. L., Luz, G. L., Witter, M. H., Godoi, R. S., Barros, G. T. (2005) Bortolotto OC Growth and yield of lettuce plants under salinity. Horticul. Brasil., Brasil. 23, 931934.

    • Search Google Scholar
    • Export Citation
  • 7.

    Azevedo, N. A. D., Tabosa, J. N. (2000) Salt stress in maize seedlings: II. Distribution of cationic macronutrients and it’s relation with sodium. Rev. Bras. Eng. Agric. Amb. 4, 165171.

    • Search Google Scholar
    • Export Citation
  • 8.

    Azoo, M. M. (2009) Foliar application of riboflavin (Vitamin B2) enhancing the resistance of Hibiscus sabdariffa L. (deep red petals variety) to salinity stress. J. Biol. Sci. 9, 109118.

    • Search Google Scholar
    • Export Citation
  • 9.

    Azooz, M. M., Shaddad, M. A., Abdel-Latef, A. A. (2004) The accumulation and compartmentation of proline in relation to salt tolerance of three sorghum cultivars. Ind. J. Plant Physiol. 9, 18.

    • Search Google Scholar
    • Export Citation
  • 10.

    Bajji, M., Lutts, S., Kient, J. M. (2001) Water deficit effects on solute contribution to osmotic adjustment as a function of leaf aging in three durum wheat (Triticum durum Defs.) cultivars performing differently in arid conditions. Plant Sci. 160, 669681.

    • Search Google Scholar
    • Export Citation
  • 11.

    Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39, 205207.

    • Search Google Scholar
    • Export Citation
  • 12.

    Bellani, L. M., Guarnieri, M., Scialabba, A. (2002) Differences in the activity and distribution of peroxidases from three different portions of germinating Brassica oleracea seed. Physiol. Plant 114, 102108.

    • Search Google Scholar
    • Export Citation
  • 13.

    Binzel, M. L., Reuveni, M. (1994) Cellular mechanisms of salt tolerance in plant cells. Hort. Rev. 16, 3370.

  • 14.

    Bor, M., Ozdemir, F., Turkan, I. (2003) The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet (Beta vulgaris L.) and wild beet (Beta maritima L.). Plant Sci. 164, 7784.

    • Search Google Scholar
    • Export Citation
  • 15.

    Cha-um, S., Kirdmanee, C. (2009) Effect of salt stress on proline accumulation, photosynthetic ability and growth characters in two maize cultivars. Pak. J. Bot. 41, 8798.

    • Search Google Scholar
    • Export Citation
  • 16.

    Cramer, G. R., Lauchli, A., Polito, V. S. (1985) Displacement of Ca from the plasmalemma of root cell. A primary response to salt stress. Plant Physiol. 79, 207211.

    • Search Google Scholar
    • Export Citation
  • 17.

    Czabator, F. J. (1962) Germination value: An index combining speed and completeness of pine seed germination. Forest Sci. 8, 386395.

  • 18.

    Dagar, J. C., Bhagwan, H., Kumar, Y. (2004) Effect on growth performance and biochemical contents of Salvadora persica when irrigated with water of different salinity. Ind. J. Plant Physiol. 9, 234238.

    • Search Google Scholar
    • Export Citation
  • 19.

    El Naim, A. M., Ahmed, S. E. (2010) Effect of weeding frequencies on growth and yield of two roselle (Hibiscus sabdariffa L.) varieties under rain fed. Aust. j. basic appl. Sci. 4, 42504255.

    • Search Google Scholar
    • Export Citation
  • 20.

    El-Sherif, M. H., Sarwat, M. I. (2007) Physiological and chemical variations in producing roselle plant (Hibiscus sabdariffa L.) by using some organic farmyard manure. World J. Agricul. Sci. 3, 609616.

    • Search Google Scholar
    • Export Citation
  • 21.

    Farag, R. S., Hallabo, S. A. S., Hewedi, F. M., Basyony, A. E. (1986) Chemical evaluator of rap seed –Feheseifen. Anstrichmi Hel. 88, 391397.

    • Search Google Scholar
    • Export Citation
  • 22.

    Farida, A. K., Das, A. B., Mittra, B., Mohanty, P. (2004) Salt-stress induced alterations in protein profile and protease activity in the mangrove (Bruguiera parvi). Z Naturforsch C. 59, 408414.

    • Search Google Scholar
    • Export Citation
  • 23.

    Fernandes, P., Cabral, J. M. S. (2007) Phytosterols: applications and recovery methods. Bioresou. Tech. 9, 23352350.

  • 24.

    Flowers, T. J., Yeo, A. R. (1989) Effects of salinity on plant growth and crop yield. In: Cherry, J. H. (Ed.). Environmental Stress in Plants. Springer-Verlag: Berlin, pp. 101119.

    • Search Google Scholar
    • Export Citation
  • 25.

    Gaspar, T., Penel, C., Castillo, F. J., Greppin, H. (2001) A two-step control of basic and acidic peroxidases and its significance for growth and development. Physiol. Plant 64, 418423.

    • Search Google Scholar
    • Export Citation
  • 26.

    Hartmann, M. A. (2004) Sterol metabolism and function in higher plants. In: Daum, G. (Ed.) Lipid Metabolism and Membrane Biogenesis. Springer-Verlag: Heidelberg, pp. 183211.

    • Search Google Scholar
    • Export Citation
  • 27.

    Heath, R., Packer, L. (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 196, 385395.

    • Search Google Scholar
    • Export Citation
  • 28.

    Hernandez, A., Almansa, M. S. (2002) Short-term effects of salt stress on antioxidant systems and leaf water deficits in leaves. Aust. J. Boil. Sci. 15, 413428.

    • Search Google Scholar
    • Export Citation
  • 29.

    Khan, M. H., Panda, S. K. (2008) Alterations in root lipid peroxidation and antioxidative responses in two rice cultivars under NaCl-salinity stress. Acta Physiol. Plant 30, 8189.

    • Search Google Scholar
    • Export Citation
  • 30.

    Laemmli, U. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680685.

  • 31.

    Levitt, J. (1980) Responses of Plants to Environmental Stresses. Vol. II, 2nd ed. Academic Press, New York, 607. p.

  • 32.

    Luna, C., Gonzalez, C., Trippi, V. (1994) Oxidative damage caused by an excess of copper in oat leaves. Plant Cell Physiol. 35, 1115.

    • Search Google Scholar
    • Export Citation
  • 33.

    Mahadevan, N., Shivali, K. P. (2009) Hibiscus sabdariffa Linn: An overview. Natural Product Radiance 8, 7783.

  • 34.

    Mandhania, S., Madan. S., Sawhney, V. (2006) Antioxidant defense mechanism under salt stress in wheat seedlings. Biol. Plant. 227, 227231.

    • Search Google Scholar
    • Export Citation
  • 35.

    Metzner, R. H., Rau, H., Senger, H. (1965) Untersuchunger zur Synchronisierbarkeit einzelner-pigment- Mangel Mutanten Von Chlorella. Planta 65, 186194.

    • Search Google Scholar
    • Export Citation
  • 36.

    Muhammad, Z., Hussain, F. (2010) Vegetative growth performance of five medicinal plants under NaCl salt stress. Pak. J. Bot. 42, 303316.

    • Search Google Scholar
    • Export Citation
  • 37.

    Munns, R., Husain, S., Rivelli, A. R., James, R. A., Condon, A. G., Lindsay, M. P., Laguda, E. S., Schachtman, D. P., Hare, R. A. (2002) Avenues for increasing salt tolerance of crops, and the role of physiologically based selection traits. Plant and Soil 247, 93105.

    • Search Google Scholar
    • Export Citation
  • 38.

    Murashige, T., Skooge, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15, 473497.

    • Search Google Scholar
    • Export Citation
  • 39.

    Reddy, M. P., Vora, A. B. (2002) Changes in pigment composition, hill reaction activity and saccharides relations of pea leaves. Physiol. Plant 115, 251257.

    • Search Google Scholar
    • Export Citation
  • 40.

    Rozbeh, F., Adel, F., Ali, A. (2015) Effect of salt stress on physiological and morphological parameters of rapeseed cultivar. J. Sci. Res. Develop. 2, 111117.

    • Search Google Scholar
    • Export Citation
  • 41.

    SAS (2003) Proc User’s Manual, Version 9.1., SAS Institute, Cary, NC, US.

  • 42.

    Savvas, D., Lenz, F. (2000) Effects of NaCl or nutrient-induced salinity on growth, yield, and composition of eggplants grown in rock wool. Scientia Horticulturae 84, 3747.

    • Search Google Scholar
    • Export Citation
  • 43.

    Sharma, P. K., Hall, D. O. (1991) Interaction of salt stress and photo inhibition on photosynthesis in barley and sorghum. J. Plant Physiol. 138, 614619.

    • Search Google Scholar
    • Export Citation
  • 44.

    Singh, P. K., Shahi, S. K., Singh, A. P. (2015) Effect of salt stress on physico-chemical changes in maize (Zea maize L.) plants in response to salicylic acid. Ind. J. Plant Sci. 4, 6977.

    • Search Google Scholar
    • Export Citation
  • 45.

    Stigmann, H., Burgermeister, W., Francksen, H., Krogerrecklen, F. (1983) Manual of gel electrophoresis and isoelectrofocusing with the apparatus Planta–Phor. Inst. Biochem., Messeweg II. D-3300 Braunschweig West Germany.

  • 46.

    Taleisnik, E., Peyrano, G., Arias, C. (1997) Response of Chloris gayana cultivars to salinity. 1. Germination and early vegetative growth. Trop. Grassl. 31, 232240.

    • Search Google Scholar
    • Export Citation
  • 47.

    Turner, N. C. (1981) Techniques and experimental approaches for the measurement of plant water status. Plant Chem. 28, 350356.

  • 48.

    Wilson, J. R., Ludlow, M. M., Fisher, M. J., Schulze, E. E. (1989) Adaptations to water stress of the leaf water relations of four tropical forage species. Aust. J. Plant Physiol. 7, 207220.

    • Search Google Scholar
    • Export Citation
  • 49.

    Zhu, J. K. (2003) Regulation of ion homeostasis under salt stress. Curr. Opin. Plant Biol. 6, 441445.

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)