View More View Less
  • 1 Jagiellonian University Department of Pharmacognosy Medyczna 9 30-688 Cracow Poland
  • | 2 Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum Medyczna 9 30-688 Cracow Poland
Restricted access

A thin layer chromatographic method with densitometric UV detection at λ = 285 nm has been developed for quantification of embelin in Lysimachia punctata . TLC analysis was performed on aluminumbacked silica gel 60 F 254 plates with n -hexane-ethyl acetate, 7 + 3 ( v / v ), as mobile phase. Under these experimental conditions the method was highly sensitive (the limit of detection was 12.5 ng) and recovery was satisfactory (from 89.45% to 92.28%). Good results obtained during validation of the method were confirmed in quantification of embelin, because high precision and accuracy were achieved.

  • H. Ogawa and S. Natori , Phytochemistry 7 (1968) 773–782.

    Natori S. , '' (1968 ) 7 Phytochemistry : 773 -782.

  • A.K. Lund, J. Lemmich, A. Adsersen , and C.E. Olsen , Phytochemistry 44 (4) (1997) 679–681.

    Olsen C.E. , '' (1997 ) 44 Phytochemistry : 679 -681.

  • G. Hussein, H. Miyashiro, N. Nakamura, M. Hattori, N. Kakiuchi , and K. Shimotohno , Phytother. Res. 14 (2000) 510–516.

    Shimotohno K. , '' (2000 ) 14 Phytother. Res. : 510 -516.

  • V.P. Dixit and S.K. Bhargava , Andrologia 15 (5) (1983) 486–494.

    Bhargava S.K. , '' (1983 ) 15 Andrologia : 486 -494.

  • O.P. Gupta, M.M. Ali, G.B.J. Ray , and C.K. Atal , Indian J. Phys. Pharm. 21 (1) (1997) 31–39.

    Atal C.K. , '' (1997 ) 21 Indian J. Phys. Pharm. : 31 -39.

  • U. Zutshi, R.K. Johri , and C.K. Atal , Indian J. Exp. Biol. 27 (7) (1989) 656–657.

    Atal C.K. , '' (1989 ) 27 Indian J. Exp. Biol. : 656 -657.

  • C.K. Atal, M.A. Siddiqui, U. Zutshi, V. Amla, R.K. Johri, P.G. Rao , and S. Kour , J. Ethnopharmacol. 11 (3) (1984) 309–317.

    Kour S. , '' (1984 ) 11 J. Ethnopharmacol. : 309 -317.

  • M. Chitra, E. Sukumar, V. Suja , and C.S. Devi , Chemotherapy 40 (2) (1994) 109–113.

    Devi C.S. , '' (1994 ) 40 Chemotherapy : 109 -113.

  • The Validation of Analytical Procedures Used in the Examination of Pharmaceutical Materials, WHO/Pharm. 89541, Rev 2 Genf, 1989.

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jan 2022 4 0 0
Feb 2022 7 0 0
Mar 2022 6 0 0
Apr 2022 6 0 0
May 2022 9 0 0
Jun 2022 4 0 0
Jul 2022 0 0 0