Among the complex mixture of biologically active compounds in the leaves of
, a plant used in traditional medicine, ursolic acid, a constituent of the leaves, has been used as an analytical marker indicative of the quality of the plant. A sensitive thin-layer chromatographic method has been developed for determination of ursolic acid in the leaves of
. Chromatographic separation was performed on silica gel HPTLC plates with toluene-ethyl acetate-triethylamine-methanol, 7 + 2 + 1 + 1 (
), as mobile phase. The plates were scanned densitometrically at
= 366 nm. The method was validated for precision, repeatability, and accuracy. Intra-day and inter-day
were 0.43% and 1.54%, respectively. Instrumental precision and repeatability of the method were found to be 0.54 and 1.20 (% CV), respectively. The accuracy was checked by studying recovery at three levels; average recovery was 100.26%. The method proposed for quantitative monitoring of ursolic acid in
is rapid, simple, and accurate and can be used for routine quality testing.
R.N. Chopra, S.L. Nayar
, Glossary of Indian medicinal plants, CSIR, New Delhi, 1956.
Chopra I.C. , '', in Glossary of Indian medicinal plants, (1956) -.
Chopra I.C. Glossary of Indian medicinal plants1956)| false
, Indian Medicinal plants. Vol. III, L.M. Basu Publications, Allahabad, 1935.
Basu B.D. , '', in Indian Medicinal plants. Vol. III, (1935) -.
Basu B.D. Indian Medicinal plants. Vol. III1935)| false
N. Valecha, C.U. Devi
, Curr. Sci.
Joshi H. , '' (2000) 78Curr. Sci.: 1120-.
Joshi H. Curr. Sci.2000781120)| false
Medicinal Plants of India, I.C.M.R., New Delhi, 1975.
, Indian J. Chem.
Banerji J. , '' (1977) 15BIndian J. Chem.: 390-391.
Banerji J. Indian J. Chem.197715B390391)| false
D.N. Dhar, S.C. Suri
, Planta Med.
Dwiwedi P. , '' (1977) 31Planta Med.: 33-34.
Dwiwedi P. Planta Med.1977313334)| false
, J. Ethnopharmacol.
Jie L. , '' (1995) 49J. Ethnopharmacol.: 57-68.
Jie L. J. Ethnopharmacol.1995495768)| false
V. Rao, V. Gopalkrishnan
K. Rajandra Prasad
, Indian Drugs
Rajandra Prasad K. , '' (2001) 38Indian Drugs: 216-224.
Rajandra Prasad K. Indian Drugs200138216224)| false
D. Baricevic, S. Sosa, D. Loggia, A. Tubaro, B. Simonovska, A. Krasna
, J. Ethnopharmacol.
Zupancic A. , '' (2001) 75J. Ethnopharmacol.: 125-132.
Zupancic A. J. Ethnopharmacol.200175125132)| false