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  • 1 Alexandria University, Egypt Department of Pharmacognosy, Faculty of Pharmacy Alexandria 21521 Egypt
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A high-performance thin layer chromatography (HPTLC) method for the simultaneous quantitative determination of diosmin and hesperidin in citrus fruit extracts and in pharmaceutical preparations was developed. Conditions were also optimized for best possible extraction of diosmin and hesperidin by using different solvents (water, methanol, and DMSO) for maximum recovery of these flavonoids. Methanol-DMSO (1:1) was found to be best for highest possible recovery of target analytes. Moreover, extraction efficiency of targeted diosmin and hesperidin from plant matrix using methanol-DMSO (1:1) were studied using cold percolation, Soxhlet, hot extraction, and ultra sonication techniques. For achieving good separation, a mobile phase of ethyl acetate-methanol-water-acetic acid (25:2:2:1, v/v/v/v) was used. The densitometric determination was carried out at 330 nm in reflection/absorption mode. The calibration curves were linear in the range of 0.1–3 μg per spot for diosmin and 0.25–7.5 μg per spot for hesperidin. Recovery was also assessed by comparison with a previously reported high-performance liquid chromatography (HPLC) method. The quantitative results of both analytical methods did not show any statistical significance between each other.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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