View More View Less
  • 1 National Institute of Chemistry, Hajdrihova 19, SI-1001 Ljubljana, Slovenia
  • | 2 National Institute of Chemistry, Hajdrihova 19, SI-1001 Ljubljana, Slovenia
Restricted access

High-performance thin-layer chromatography (HPTLC) is a powerful separation technique which is often overlooked. In this study, we comprehensively assessed the applicability, ease, and performance of HPTLC in combination with densitometry and mass spectrometry (MS) to characterize physalins — relatively polar secondary metabolites from Physalis alkekengi L. HPTLC silica gel plates were evaluated in combination with 14 developing solvents (13 published in the literature). Bonded stationary phases (HPTLC RP-18, RP-18 W, CN F254S) were also tested. Four detection reagents (sulfuric acid, anisaldehyde, 4-dimethylaminocinnamaldehyde (DMACA), and molybdatophosphoric acid) were compared to ascertain which one is the most suitable. For all chromatographic analyses, a commercial standard physalin L and a P. alkekengi L. crude extract were used. In some cases, physalin L standard appeared as two clearly resolved bands on silica plates, but only after derivatization, where sulfuric acid reagent provided the best selectivity and sensitivity. Physalin L standard impurity was found to belong to the physalin family as confirmed by HPTLC–MS/(MS) and nuclear magnetic resonance (NMR) spectroscopy. Compared to high-performance liquid chromatography (HPLC), our HPTLC method showed extremely high sensitivity for standard impurity (ca. 4% determined by NMR) as it was clearly visible on the plate during image analysis after derivatization. Unlike (ultra)-high-performance liquid chromatography ((U)HPLC), HPTLC was also able to separate physalin L standard from its impurity. We show that (HP)TLC is a suitable chromatographic technique for the analysis of physalins and can even surpass the performance of (U)HPLC, namely, due to a wide array of different developing solvents available.

  • [1]

    A.R. Gohari , S. Saeidnia, A. Hadjiakhoondi, M. Sharifzadeh, M.R. Gohari, Pharmacologyonline 3 (2008) 724729.

  • [2]

    J. Estakhr , N. Sanchooli, Sh. Najafi, N. Javdan, Res. J. Pharm. Biol. Chem. Sci. 2 (2011) 421425.

  • [3]

    H. Kang , S-R. Kwon, H-Y. Choi, J. Ethnopharmacol. 135 (2011) 95101.

  • [4]

    X. Li , C. Zhang, W. Li, D. Wu, J. Liu, L. Tang, Y. Xin, Fitoterapia 87 (2013) 4348.

  • [5]

    M. Kawai , T. Matsuura, S. Kyuno, H. Matsuki, M. Takenaka, T. Katsuoka, Y. Butsugan, K. Saito, Phytochemistry 26 (1987) 33133317.

  • [6]

    B. Makino , M. Kawai, K. Kito, H. Yamamura, Y. Butsugan, Tetrahedron 51 (1995) 1252912538.

  • [7]

    Y. Zheng , L. Luan, Y. Chen, Y. Ren, Y. Wu, J. Pharm. Biomed. Anal. 71 (2012) 5462.

  • [8]

    C. Huang , Q. Xu, C. Chen, C. Song, Y. Xu, Y. Xiang, Y. Feng, Q. Ouyang, Y. Zhang, H. Jiang, J. Chromatogr. A 1361 (2014) 139152.

  • [9]

    P. Weller , D.E. Breithaupt, J. Agric. Food Chem. 51 (2003) 70447049.

  • [10]

    V.I. Deineka , V.N. Sorokopudov, L.A. Deineka, M.Y. Tret’yakov, V.V. Fesenko, Pharm. Chem. J. 42 (2008) 8788.

  • [11]

    A. Pintea , A. Varga, P. Stepnowski, C. Socaciu, M. Culea, H.A. Diehl, Phytochem. Anal. 16 (2005) 188195.

  • [12]

    K. Basey , J.G. Woolley, Phytochemistry 12 (1973) 25572559.

  • [13]

    K. Basey , B.A. McGaw, J.G. Woolley, Phytochemistry 31 (1992) 41734176.

  • [14]

    E. Kranjc , A. Albreht, I. Vovk, D. Makuc, J. Plavec, J. Chromatogr. A 1437 (2016) 95106.

  • [15]

    Y. Zheng , Y. Chen, Y. Ren, L. Luan, Y. Wu, Phytochem. Anal. 23 (2012) 337344.

  • [16]

    B. Xu , C. Ju, H. Guan, L. Xu, N. Xu, B. Wang, J. Med. Plants Res. 6 (2012) 24382442.

  • [17]

    Y. Zheng , Y. Chen, Y. Ren, L. Luan, Y. Wu, J. Pharm. Biomed. Anal. 58 (2012) 94101.

  • [18]

    Y. Zheng , J. Chen, L. Liu, X. Liang, D. Hong, Biomed. Chromatogr. 30 (2016) 12781284.

  • [19]

    J. Sherma , J. Strock, in: T. Kowalska, M. Sajewicz, J. Sherma (eds.), Planar Chromatography –Mass Spectrometry, Chromatographic Science Series, Vol. 110, CRC Press, Taylor & Francis Group, Boca Raton, FL, 2016, pp.3558.

    • Search Google Scholar
    • Export Citation
  • [20]

    V. Glavnik , I. Vovk, A. Albreht, J. Chromatogr. A 1482 (2017) 97108.

  • [21]

    S. Helvacı , G. Kökdil, M. Kawai, N. Duran, G. Duran, A. Güvenç, Pharm. Biol. 48 (2010) 142150.

  • [22]

    G. Sen , H.D. Pathak, Phytochemistry 39 (1995) 12451246.

  • [23]

    M. Kawai , T. Yamamoto, B. Makino, H. Yamamura, S. Araki, Y. Butsugan, K. Saito, J. Asian Nat. Prod. Res. 3 (2001) 199205.

  • [24]

    A. Khoshlahjeh , D.E. Asli, Z. Lotfi, Z.F. Kashani, M. Shirmard, Int. J. Biosci. 3 (2013) 204211.

  • [25]

    C.C. Lee , P. Houghton, J. Ethnopharmacol. 100 (2005) 237243.

  • [26]

    A.H. Januário , E. Rodrigues Filho, R.C.L.R. Pietro, S. Kashima, D.N. Sato, S.C. França, Phytother. Res. 16 (2002) 445448.

  • [27]

    A.G. Damu , P.C. Kuo, C.R. Su, T.H. Kuo, T.H. Chen, K.F. Bastow, K.H. Lee, T.S. Wu, J. Nat. Prod. 70 (2007) 11461152.

  • [28]

    H. Jork , W. Funk, W. Fischer, H. Wimmer, Dünnschicht-Chromatographie. Reagenzien und Nachweismethoden, Bd. 1a, Physikalishe und chemische Nachweismethoden: Grundlagen, Reagenzien I, VCH Verlagsgesellschaft, Weinheim, 1989, pp.195198, 342–345, 402–406.

    • Search Google Scholar
    • Export Citation
  • [29]

    V. Glavnik , B. Simonovska, I. Vovk, J. Chromatogr. A 1216 (2009) 44854491.

  • [30]

    J. Sherma , F. Rabel, LCGC North Am. 30 (2012) 458472.

  • [31]

    L.R. Snyder , J. Chromatogr. A 656 (1993) 537547.

For Submission Guideline click HERE

Manuscript Submission: JPC Manuscript Submission

Senior editors

Editor(s)-in-Chief: B. Spangenberg

Managing Editor: Kakuk Ágnes

Editorial Board

  • T.H. Dzido (Lublin, Poland)
  • Á. M. Móricz (Budapest, Hungary)
  • C.F. Poole (Detroit, MI, USA)
  • E. Reich (Muttenz, Switzerland)
  • B. Renger (Radolfzell, Germany)
  • I. Vovk (Ljubljana, Slovenia)

 

Advisory Board

  • E. BODOKI (Cluj-Napoca, Romania)
  • U. A. TH. BRINKMAN (Amsterdam, The Netherlands)
  • I. CHOMA (Lublin, Poland)
  • V. COMAN (Cluj-Napoca, Romania)
  • W. DAMMERTZ (Kressbronn, Germany)
  • K. FERENCZI-FODOR (Budapest, Hungary)
  • G. INDRAYANTO (Surabaya, Indonesia)
  • R. E. KAISER (Bad Dürkheim, Germany)
  • I. KLEBOVICH (Budapest, Hungary)
  • T. KOWALSKA (Katowice, Poland)
  • L. LEPRI (Florence, Italy)
  • E. MINCSOVICS (Budapest, Hungary)
  • D. NUROK (Indianapolis, IN, USA)
  • B. RENGER (Radolfzell, Germany)
  • J. K. RÓŻYŁO (Lublin, Poland)
  • J. SHERMA (Easton, PA, USA)
  • M. WAKSMUNDZKA-HAJNOS (Lublin, Poland)
  • D. WEDGE (Mississippi, USA)

A. Kakuk - Managing Editor, Akadémiai Kiadó
Address: P.O. Box 245. H-1519 Budapest, Hungary
Fax: +36 1 464 82 95
E-mail: jpc@akkrt.hu

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences

 

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2021 5 0 0
Jul 2021 1 0 0
Aug 2021 6 0 0
Sep 2021 2 0 0
Oct 2021 5 0 0
Nov 2021 3 0 0
Dec 2021 0 0 0