Presently, sterols like stigmasterol, β-sitosterol, campesterol, and ergosterol are identified and quantified from leaves, stem, and inflorescence of Heteropogon contortus. (L.) Beauv (family: Poaceae) from Rajasthan, India, using high-performance thin-layer chromatography (HPTLC). H. contortus is reported with various medicinal properties. In the present study, a HPTLC–densitometric method was developed for the quantification of sterols by using toluene‒methanol‒formic acid (9:1:0.3 v/v) as the mobile phase. Post-derivatization of the plates was done by using freshly prepared anisaldehyde–sulfuric acid, heated at 110–120°C and scanned at 530 nm absorbing wavelength. The maximum contents of stigmasterol, campesterol, and ergosterol were estimated in inflorescence extracts (11.64 ± 017, 19.90 ± 0.18, and 16.64 ± 0.81 mg g−1 of dry wt. w/w, respectively), whereas β-sitosterol was found maximum in leaves, i.e., 56.52 ± 1.25 mg g−1 of dry wt (w/w). This HPTLC method is found to be simple, precise, specific, sensitive, and accurate. It can be easily used in the routine quality control of raw materials.
J.D.Blake, G.N.Richards, Aust. J. Chem.23 (1970) 2353–2360.
J.D.Blake, G.N.Richards, Aust. J. Chem.23 (1970) 2353–2360.)| false
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