Acta Chromatographica
Volume/Issue:
Volume 20: Issue 3
Development and validation of a stability-indicating HPTLC method for analysis of 3-acetyl-11-keto-β-boswellic acid in a herbal extract and a nanoparticles formulation
Authors:
A. Goel Central Indian Pharmacopoeia Laboratory, Ministry of Health and Family Welfare, Government of India, Ghaziabad, Uttar Pradesh, India

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R. Goel Department of Pharmaceutics, Jamia Hamdard, Hamdard University, New Delhi, India

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G. Jain Jain Department of Pharmaceutics, Jamia Hamdard, Hamdard University, New Delhi, India

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R. Singh Singh Central Indian Pharmacopoeia Laboratory, Ministry of Health and Family Welfare, Government of India, Ghaziabad, Uttar Pradesh, India

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F. Ahmad Ahmad Department of Pharmaceutics, Jamia Hamdard, Hamdard University, New Delhi, India

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G. Singh Singh Central Indian Pharmacopoeia Laboratory, Ministry of Health and Family Welfare, Government of India, Ghaziabad, Uttar Pradesh, India

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Pages:
497–511
Online Publication Date:
01 Sep 2008
Publication Date:
01 Sep 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.20.2008.3.15
Keywords:
3-acetyl-11-keto-β-boswellic acid; high-performance thin layer chromatography; method validation; stability-indicating
Open access

Summary

A new, specific, sensitive, selective, precise, and reproducible high-performance thin-layer chromatographic (HPTLC) method has been established for study of the stability of 3-acetyl-11-keto-β-boswellic acid (AKBA). HPTLC was performed on aluminium foil plates coated with 200 μm silica gel 60F254. Linear ascending development with toluene-ethyl acetate 7:3 (v/v) was performed at room temperature (25 ± 2°C) in a twin-trough glass chamber saturated with mobile phase vapour. Compact bands (RF 0.52 ± 0.02) were obtained for AKBA. Spectrodensitometric scanning was performed in absorbance mode at 250 nm. Linear regression analysis of the calibration plots showed there was a good linear relationship (r2 = 0.9989 ± 0.0002) between peak area and concentration in the range 200–1200 ng band−1. The method was validated for precision, recovery, robustness, specificity, and detection and quantification limits, in accordance with ICH guidelines. The limits of detection and quantification were 3.06 and 9.29 ng band−1, respectively. The recovery of the method was 99.35–100.21%. AKBA was subjected to various stress test conditions — acid and alkali hydrolysis, oxidation, photodegradation, and dry and wet heat treatment. Degradation products were well resolved from the pure drug with significantly different RF values. Statistical analysis showed the method could be successfully applied for the estimation of AKBA in herbal extract and in nanoparticles. Because the method could effectively separate the drug from its degradation products, it can be regarded as stability-indicating.

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Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736
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Senior editors

Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Yvan Vander Heyden, Free University of Brussels, Brussels, Belgium
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Brata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

KOWALSKA, TERESA (1946-2023)
E-mail: kowalska@us.edu.pl

SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl

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2023  
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Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
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Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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