Acta Chromatographica
Volume/Issue:
Volume 21: Issue 1
Improved high-performance liquid chromatography-DAD method for the simultaneous analysis of quercetin and kaempferol in the stems of Cissus quadrangularis Linn.
Authors:
A. K. Thakur 1A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division, University Institute of Chemical Technology, Nathalal Parikh Marg, Matunga, Mumbai, 400 019, Maharastra, India

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V. Jain 1A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division, University Institute of Chemical Technology, Nathalal Parikh Marg, Matunga, Mumbai, 400 019, Maharastra, India

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L. Hingorani Pharmanza Herbal Pvt. Ltd, Plot no 214, Near Vadadia Patiya, Borsad-Tarapur Road, Kaniya, 388435, Dist. Anand, Gujrat, India

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K. S. Laddha 1A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division, University Institute of Chemical Technology, Nathalal Parikh Marg, Matunga, Mumbai, 400 019, Maharastra, India

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Pages:
95–103
Online Publication Date:
01 Mar 2009
Publication Date:
01 Mar 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.21.2009.1.8
Keywords:
quantitative HPLC; quercetin; kaempferol
Open access

Summary

A simple, rapid, and specific reversed-phase HPLC method with DAD detection has been used for analysis of two flavonoids, quercetin and kaempferol, in Cissus quadrangularis Linn. The flavonoids were well resolved within 10 min, and quantification was achieved, on an endcapped C18 column at 370 nm with acetonitrile-phosphate buffer (pH 3.4, adjusted with glacial acetic acid) 60:40 (v/v) as isocratic mobile phase at a flow rate of 1.0 mL min−1. The method was validated for limit of detection, limit of quantification, linearity, precision, accuracy, and recovery. Linearity was demonstrated over the range 0.75 to 10 μg mL−1 for quercetin and 1.0 to 10 μg mL−1 for kaempferol with good correlation coefficients (r2 > 0.998). Detection limits were 0.075 and 0.10 μg mL−1 for quercetin and kaempferol respectively. Recovery was 98.0–105.3% for quercetin and 95.0–101.1% for kaempferol. The method was successfully applied to analysis of two flavonoids in four samples of Cissus quadrangularis Linn.

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Acta Chromatographica
Online ISSN:
2083-5736
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Senior editors

Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Yvan Vander Heyden, Free University of Brussels, Brussels, Belgium
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Brata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

KOWALSKA, TERESA (1946-2023)
E-mail: kowalska@us.edu.pl

SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl

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2023  
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Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
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CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
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Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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