Acta Chromatographica
Volume/Issue:
Volume 22: Issue 1
Validated HPLC method development for simultaneous analysis of withaferin-A and 6-gingerol
Authors:
V. Jain A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga (E), Mumbai, India, 400 019

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A. Thakur A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga (E), Mumbai, India, 400 019

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G. Soman Nisarga Biotech Pvt. Ltd, 275 Chandan Nagar, Addl. MIDC, Satara, India, 415 004

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K. S. Laddha A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga (E), Mumbai, India, 400 019

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Pages:
153–159
Online Publication Date:
01 Mar 2010
Publication Date:
01 Mar 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.22.2010.1.12
Keywords:
HPLC; simultaneous analysis; polyherbal formulation; withaferin-A; 6-gingerol
Open access

Summary

A simple, rapid, and specific reversed-phase HPLC method has been developed for simultaneous analysis of withaferin-A and 6-gingerol in a polyherbal formulation containing Withania somnifera and Zingiber officinalis extracts. HPLC analysis was performed on a C18 column using a 40:60 (v/v) mixture of acetonitrile and water as isocratic mobile phase at a flow rate of 1.5 mL min−1. UV detection was at 227 nm for withaferin-A and 278 nm for 6-gingerol. The method was validated for accuracy, precision, linearity, specificity, and sensitivity in accordance with International Conference on Harmonization guidelines. Validation revealed the method is specific, accurate, precise, reliable and reproducible. Good linear correlation coefficients (r2 > 0.9996) were obtained for calibration plots in the ranges tested. Limits of detection were 0.2 and 0.4 μg and limits of quantification were 0.5 and 1.0 μg for withaferin-A and 6-gingerol, respectively. Intra and inter-day RSD of retention times and peak areas were less than 2.1%. Recovery was between 94.5 and 98.8% for withaferin-A and 94.2 and 102.4% for 6-gingerol. The established HPLC method is appropriate and the two markers are well resolved, enabling efficient quantitative analysis of withaferin-A and 6-gingerol. The method was successfully used for quantitative analysis of these two marker constituents in a marketed polyherbal formulation.

  • [1]. S.K. Kulkarni B. George R. Mathur 1991 Phytother. Res. 12 451–453.

  • [2]. P.U. Devi 1996 Indian J. Exp. Biol. 34 927–932.

  • [3]. S.K. Bhattacharya K.S. Satyan S. Ghosal 1997 Indian J. Exp. Biol. 35 236–239.

  • [4]. S. Chrubasik M.H. Pittler B.D. Roufogalis 2005 Phytomedicine 12 684–701.

  • [5]. P.N. Ravindran K.N. Babu 2004 Ginger: the Genus Zingiber. Medicinal and Aromatic Plants — Industrial Profiles CRC Press Florida 431–434.

    • Search Google Scholar
    • Export Citation

  • [6]. A.P. Gupta R.K. Verma H.O. Misra M.M. Gupta 1996 J. Med. Aromat. Plant Sci. 18 788–790.

  • [7]. S. Dalavayi S.M. Kulkarni R.J. Itikala S. Itikala 2006 Indian J. Pharm. Sci. 68 253–256.

  • [8]. V. Ciddi 2006 Indian J. Pharm. Sci. 68 490–492.

  • [9]. R.S. Sangwan , N.D. Chaurasiya, L.N. Misra, P. Lal, G.C. Uniyal, N.S. Sangwan, A.K. Srivastava, K.A. Suri, G.N. Qazi, and R. Tuli, United States Patent 7108870, 2005.

  • [10]. H.A. Schwertner D.C. Rios 2007 J. Chromatogr. B 856 41–47.

  • [11]. S. Lee C. Khoo C.W. Halstead T. Huynh A. Bensoussan 2007 J. AOAC Int. 90 1219–1226.

  • [12]. M.S. Baliga G.C. Jagetia P. Venkatesh R. Reddy J.N. Ulloor 2004 Br. J. Radiol. 77 1027–1035.

  • [13]. G.C. Jagetia T.K. Reddy K.J. Malagi B.S. Nayak M.B.R. Naidu P.B. Ravikiran S.U. Kamath P.C. Shetty D.S. Reddy 2005 Phytother. Res. 19 772–778.

    • Search Google Scholar
    • Export Citation
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Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

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2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
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CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
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SJR Q rank Q3

Acta Chromatographica
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Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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