Acta Chromatographica
Volume/Issue:
Volume 22: Issue 3
Validated HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula Linn.
Authors:
S. G. Bhope Tulip Lab Pvt Ltd, F-20/21, MIDC Ranjangaon, Pune, 412220, India

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V. V. Kuber Tulip Lab Pvt Ltd, F-20/21, MIDC Ranjangaon, Pune, 412220, India

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D. H. Nagore Tulip Lab Pvt Ltd, F-20/21, MIDC Ranjangaon, Pune, 412220, India

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Pages:
481–489
Online Publication Date:
01 Sep 2010
Publication Date:
01 Sep 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.22.2010.3.11
Keywords:
HPTLC; sennoside A; sennoside B; kaempferol
Open access

Summary

This paper describes a simple, precise, and accurate HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula whole plant extract. Chromatographic separation of the sample extract was performed on aluminium foil plates coated with silica gel 60 F254 as stationary phase. The mobile phase was toluene-ethyl acetate-methanol-formic acid 8:10:5:2 (υ/υ). Densitometric evaluation of the separated bands was performed at 270 nm. Sennosides A and B and kaempferol were satisfactorily resolved at RF 0.22 ± 0.05, 0.19 ± 0.05, and 0.81 ± 0.05, respectively. Recovery of sennosides A and B and kaempferol from Cassia fistula extract was 98.03, 98.74, and 99.08%, respectively. The method was validated for specificity, accuracy, linearity (100–400 ng per band), and precision (instrument precision in the range 1.03–1.33 and method precision in the range of 1.31–1.75) in accordance with ICH guidelines.

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Acta Chromatographica
Online ISSN:
2083-5736
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Senior editors

Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Yvan Vander Heyden, Free University of Brussels, Brussels, Belgium
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Brata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

KOWALSKA, TERESA (1946-2023)
E-mail: kowalska@us.edu.pl

SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl

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2023  
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Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
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Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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