A simple high-performance anion-exchange chromatography-integrated pulsed amperometric detection method was developed to determine the gammaaminobutyric acid (GABA) content in mulberry. GABA was separated in anion-exchange column under isocratic condition of 20 mM sodium hydroxide solution and detected at integrated pulsed amperometric detection at pico-gram levels. The contents of GABA in the leaf, stem, and root bark of mulberry were calculated without any derivatization or sample purification process using this method. This method showed good linearity, precision, and accuracy for GABA analysis.
[1]. N. Inomata T. Ishihara N. Akaike 1988 Br. J. Pharmacol. 93 679.
[2]. T. Norikura A. Kojima-Yuasa D. O. Kennedy I. Matsui-Yuasa 2007 Amino Acids 32 419.
[3]. D. Brioni A.H. Nagahara J.L. McGaugh 1989 Brain Res. 487 105.
[4]. A.D. Genazzani M. Stomati C. Bersi S. Luisi M. Fedalti M. Santuz G. Esposito F. Petraglia A.R. Genazzani 2000 J. Endocrinol. Invest. 23 526.
[5]. P.P. Vescovi R. Volpi V. Coiro 1998 Alcohol 16 325.
[6]. J.R. Menzies M. Ludwig G. Leng 2010 J. Neuroendocrinol. 22 585.
[7]. P. Reisi H. Alaei S. Babri M.R. Sharifi G. Mohaddes E. Soleimannejad B. Rashidi 2010 J. Res. Med. Sci. 15 172.
[8]. F. Sherif S. Ahmed 1995 Clin. Biochem. 28 145.
[9]. S. Lim K. Kim J. Do 2009 Korean J. Food Sci. Anim. Resour. 29 15.
[10]. G. Clarke S. O'Mahony G. Malone T. Dinan 2007 J. Neurosci. Methods 160 223.
[11]. L. Zhang M. Sun 2005 J. Chromatogr. A 1095 185.
[12]. K. Buck P. Voehringer B. Ferger 2009 J. Neurosci. Methods 182 78.
[13]. A. Ishikawa H. Oka M. Hiemori H. Yamashita M. Kimoto H. Kawasaki H. Tsuji 2009 J. Nutr. Sci. Vitaminol. 55 292.
[14]. K.Y. Syu C.L. Lin H.C. Huang J.K. Lin 2008 J. Agric. Food Chem. 56 7637.
[15]. W. LaCourse D. Johnson 1993 Anal. Chem. 65 50.
[16]. Y. Genzel S. König U. Reichl 2004 Anal. Biochem. 335 119.
[17]. H. Yu Y. Ding S. Mou P. Jandik J. Cheng 2002 J. Chromatogr. A 966 89.
[18]. P. Jandik J. Cheng N. Avdalovic 2004 J. Biochem. Biophys. Methods 60 191.
[19]. J.S. Jeong H.J. Kwon Y.M. Lee H.R. Yoon S.P. Hong 2007 J. Chromatogr. A 1164 167.
[20]. H.S. Bang W.C. Lee H.R. Shon Y.C. Choi H.B. Kim 1998 Int. J. Ind. Entomol. 40 13.
[21]. S.K. Yoo M.J. Kim J.W. Kim S.J. Rhee 2002 J. Korean Soc. Food Sci. Nutr. 31 1071.