A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of glycyrrhizin, formononetin, glycyrrhetinic acid, liquiritin, isoliquiritigenin, and licochalcone A in licorice. An Eclipse Plus C18 column (I.D. 4.6 × 100 mm, 3.5 μm particle size; Agilent) was used in the analysis. Electrospray ionization (ESI)-tandem interface in the negative mode was performed, and multiple reaction monitoring (MRM) was employed with the precursor multiple reaction monitoring production combination for the determination of six analytes. The average recoveries ranged from 98.30% to 100.13% with relative standard deviations (RSDs) ≤ 1.95%, and limits of detection (LODs) ranged from 2.1 to 3.6 pg. The applicability of this analytical approach was confirmed by the successful analysis of six samples. The results indicated that the established method was validated, sensitive, and reliable for the determination of six analytes in licorice.
[1]. C. Fiore M. Eisenhut E. Ragazzi G. Zanchin D.J. Armanini 2005 Ethnopharmacology 99 317–324.
[2]. E. Ibanoglu S. Ibanoglu 2000 Food Chem. 70 333–336.
[3]. R. Amarowicza , R.B. Pegg, P. Rahimi-Moghaddam, B. Barld, and J.A. Weilc, Food Chem. 84, 551–562 (2004).
[4]. The dictionary of traditional Chinese medicine, 2nd edn. (2006) Scientific & Technical Publishers, Shanghai, 788–789.
[5]. P. Kalaiarasi K. Viswanathan Pugalendi 2009 Eur. J. Pharmacol. 606 269–273.
[6]. P. Kalaiarasi K. Kaviarasan K. Viswanathan Pugalendi 2009 Eur. J. Pharmacol. 612 93–97.
[7]. W. Wang X. Hu Z. Zhao P. Liu Y. Hu J. Zhou D. Zhou Z. Wang D. Guo H. Guo 2008 Prog. Neuropsychopharmacol. Biol. Psychiatry. 32 1179–1184.
[8]. M. Kimura T. Moro H. Motegi H. Maruyama M. Sekine H. Okamoto H. Inoue T. Sato M. Ogihara 2008 Eur. J. Pharmacol. 579 357–364.
[9]. L.C. Mishra A. Bhattacharya V.K. Bhasin 2009 Acta Trop. 109 194–198.
[10]. B. Liu J. Yang Q. Wen Y. Li 2008 Eur. J. Pharmacol. 587 257–266.
[11]. H. Mu Y.H. Bai S.T. Wang Z.M. Zhu Y.W. Zhang 2009 Phytomedicine 16 314–319.
[12]. I.S. Lurie S.G. Toske 2008 J. Chromatogr., A 1188 322–326.
[13]. J.B. Xie Y.Q. Zhang D.Q. Kong M. Rexit 2011 J Food Compos. Anal. 24 1069–1072.
[14]. M. Giorgi A. Meizler P.C. Mills 2012 J. Pharm. Biomed. Anal. 67–68 494–500.
[15]. Y.L. Hu Y. Mechref 2012 Electrophoresis 33 1768–1777.
[16]. Q. Zhang M. Ye 2009 J. Chromatogr., A 1216 1954–1969.
[17]. S.F. Cui B.Q. Fu F. L. Sen-Chun X.R. Wang 2005 J. Chromatogr., B 828 33–40.
[18]. F. Rauchensteiner Y. Matsumura Y. Yamamotoa S. Yamaji T.J. Tani 2005 Pharm. Biomed. Anal. 38 594–600.
[19]. H. Li B.T. Chen L. Liu Q. Liu 2009 Chromatographia 69 229–235.
[20]. W.C. Liao Y.H. Lin T.M. Chang W.Y. Huang 2012 Food Chem. 132 2188–2193.
[21]. J.R. Hennell S. Lee C.S. Khoo M.J. Gray A. Bensoussan 2008 J. Pharm. Biomed. Anal. 47 494–500.
[22]. Y.Q. Zhang , J.B. Xie, Y. Liu, and D.P. Wang, Anal. Lett. 43, 2210–2219 (2010).