Authors:
Esmaiel AmraieIsfahan University of Medical Sciences, Isfahan, Iran

Search for other papers by Esmaiel Amraie in
Current site
Google Scholar
PubMed
Close
,
Masome Khosravi FarsaniIsfahan University of Medical Sciences, Isfahan, Iran

Search for other papers by Masome Khosravi Farsani in
Current site
Google Scholar
PubMed
Close
,
Leila SadeghiTarbiat Modares University, Tehran, Iran

Search for other papers by Leila Sadeghi in
Current site
Google Scholar
PubMed
Close
,
Tayaba Naim KhanMBA Institute of Business Management, Karachi, Pakistan

Search for other papers by Tayaba Naim Khan in
Current site
Google Scholar
PubMed
Close
,
Vahid Yousefi BabadiPayam Noor University of Isfahan, Isfahan, Iran

Search for other papers by Vahid Yousefi Babadi in
Current site
Google Scholar
PubMed
Close
, and
Zohrab AdaviPayam Noor University of Isfahan, Isfahan, Iran

Search for other papers by Zohrab Adavi in
Current site
Google Scholar
PubMed
Close
Open access

Diabetes is a common metabolic disorder that is specified by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The use of nonpharmacological treatments (herbal agents) is a new approach in the management of diabetes. The aim of this study was to investigate the effect of aqueous extract of alfalfa on blood glucose and serum lipids in alloxan-induced diabetic rats. In this study, 32 female rats (210–250 g) were used which were divided randomly into 4 groups including intact control group, diabetic control group, and 2 diabetic groups which received 250 and 500 mg/kg doses of aqueous extract of alfalfa, respectively. In the diabetic groups, alloxan-monohydrate was injected peritoneally to create diabetic condition. The two last groups orally received aqueous extract of alfalfa for 21 days. At the end of experiment, sugar, cholesterol, triglycerides, high-density and low-density lipoprotein, and aspartate aminotransferase (ALT) and alanine aminotransferase (AST) levels were measured in the samples. Consumption of aqueous alfalfa extract significantly reduced glucose, cholesterol, triglycerides, and low-density lipoprotein (LDL) levels in the diabetic rats but enhanced high-density lipoprotein (HDL) levels. ALT and AST liver enzyme levels were also reduced in blood. Histological examination showed that the aqueous alfalfa extract caused reconstruction of damaged liver and enhanced Langerhans islets’ diameter in pancreas. Therefore, all signs of diabetes were improved by oral administration of alfalfa in defined dose.

  • 1.

    Shaw JE , Zimmet PZ: Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 87, 414 (2010)

  • 2.

    Cole JB , Stellpflug SJ, Ellsworth H, Anderson CP, Adams AB, Engebretsen KM, Holger JS: A blinded, randomized, controlled trial of three doses of high-dose insulin in poison-induced cardiogenic shock. Clin Toxicol (Phila) 51, 201207 (2013)

    • Search Google Scholar
    • Export Citation
  • 3.

    Isah AB , Ibrahim YK, Abdulrahman EM, Ibrahim MA: The hypoglycaemic activity of the aqueous extract of Stachytarphetaangustifoli (Verbanaceae) in normoglycaemic and alloxan induced diabetic rats. Pak J Biol Sci 10, 137141 (2007)

    • Search Google Scholar
    • Export Citation
  • 4.

    Elakovich SD , Hampton JM: Analysis of coumestrol, phytoestrogens, in alfalfa tablets sold for human consumption. J Agric Food Chem 32, 173175 (1988)

    • Search Google Scholar
    • Export Citation
  • 5.

    Zargari A (1996): Therapeutic Plants, 1st ed. Tehran University Press, Tehran, pp. 642646

  • 6.

    Hong YH , Chao WW, Chen ML, Lin BF: Ethyl acetate extracts of alfalfa (Medicago sativa L.) sprouts inhibit lipopolysaccharideinduced inflammation in vitro and in vivo. J Biomed Sci 10, 1664 (2009)

    • Search Google Scholar
    • Export Citation
  • 7.

    Asgary S , Moshtaghian J, Hosseini M, Siadat H: Effects of alfalfa on lipoproteins and fatty streak formation in hypercholesterolemic rabbits. Pak J Pharm Sci 21, 460464 (2008)

    • Search Google Scholar
    • Export Citation
  • 8.

    Mehranjani MS , Shariatzadeh MA, Desfulian AR, Noori M, Abnosi MH, Moghadam ZH: Effects of Medicago sativa on nephropathy in diabetic rats. Indian J Pharm Sci 69, 768772 (2007)

    • Search Google Scholar
    • Export Citation
  • 9.

    Lust JB (1986): The Herb Book. Bantam Books Inc., London

  • 10.

    Gray AM , Flatt PR: The traditional plant treatment, Sambucus nigra (elder), exhibits insulin-like and insulin-releasing actions in vitro. J Nutr 78, 325334 (1997)

    • Search Google Scholar
    • Export Citation
  • 11.

    Winiarska H , Dworacka M, Borowska M, Bobkiewicz-Kozlowska T, Gorecki P: The effects of plant extracts of Medicago sativa and Trigonella foenum-graceum on postprandial glucose levels in type 2 diabetic rats. Herba Polonica 53, 3444 (2007)

    • Search Google Scholar
    • Export Citation
  • 12.

    Gokce G , Haznedaroglu MZ: Evaluation of antidiabetic, antioxidant and vasoprotective effects of posidonia oceaninca extract. J Ethnopharmacol 115, 122130 (2008)

    • Search Google Scholar
    • Export Citation
  • 13.

    Udayakumar R , Kasthurirengan S, Mariashibu TS, Rajesh M, Anbazhagan VR, Kim SC, Ganapathi A, Choi CW: Hypoglycemic and hypolipidaemic effects of Withania somnifera root and leaf extracts on alloxan-induced diabetic rats. Int J Mol Sci 10, 23672382 (2009)

    • Search Google Scholar
    • Export Citation
  • 14.

    Dhandapani S , Subramanian VR, Rajagopal S, Namasivayam N: Hypolipidemic effects of Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacol Res 46, 251255 (2002)

    • Search Google Scholar
    • Export Citation
  • 15.

    Matkovics B , Sasvári M, Kotormán M, Varga IS, Hai DQ, Varga C: Further prove on oxidative stress in alloxan diabetic rat tissues. Acta Physiol Hung 85, 183192 (1997)

    • Search Google Scholar
    • Export Citation
  • 16.

    Takasu N , Asawa T, Komiya I, Nagasawa Y, Yamada T: Alloxan-induced DNA strand breaks in pancreatic islets. Evidence for H2O2 as an intermediate. J Biol Chem 266, 21122114 (1991)

    • Search Google Scholar
    • Export Citation
  • 17.

    Hyder MZ : Comparative levels of ALT, AST, ALP and GGT in liver associated diseases. Am J Clin Pathol 28, 5662 (1957)

  • 18.

    Cnop M , Welsh N, Jonas JC, Jörns A, Lenzen S, Eizirik DL: Mechanisms of pancreatic cell death in type 1 and type 2 diabetes. Diabetes 54, S97S107 (2005)

    • Search Google Scholar
    • Export Citation
  • 19.

    Devi PU , Sharada AC, Solomon FE: Anti-tumor and radiosensitizing effects of Withania somniferra (Ashwagadha) on a transplantable mouse tumor sarcoma. J Exp Biol 31, 607611 (1993)

    • Search Google Scholar
    • Export Citation
  • 20.

    Colodny LR : Alfalfa saponins may help reduce cholesterol levels. J Am Nut Ass 3, 110 (2001)

  • 21.

    Jackson IM : Abundance of immunoreactive thyrotropin-releasing hormone-like material in the alfalfa plant. Endocrinol 108, 344346 (1981)

    • Search Google Scholar
    • Export Citation
  • 22.

    Barens DK , Sheaffer C: Alfalfa. Agriculture 2, 205216 (1995)

  • 23.

    Concepción Navarro M , Pilar Montilla M, Martín A, Jiménez J, Pilar Utrilla M: Free radical scavenger and antihepatotoxic activity of rosmarinus. Plant Med 59, 312314 (1993)

    • Search Google Scholar
    • Export Citation
  • 24.

    Prince P , Menon VP: Hypoglycemic and other related actions of Tinospora cardifolia roots in Alloxan-induced diabetic rats. J Etnopharmocal 70, 915 (2000)

    • Search Google Scholar
    • Export Citation
  • 25.

    McClenaghan NH , Barnett CR, Ah-Sing E, Abdel-Wahab YH, O’Harte FP, Yoon TW, Swanston-Flatt SK, Flatt PR: Characterization of a novel glucose-responsive insulin-secreting cell line, BRIN-BD11, produced by electrofusion. Diabetes 45, 11321140 (1996)

    • Search Google Scholar
    • Export Citation
  • 26.

    Rubenstein AH , Levin NW, Elliott GA: Manganese-induced hypoglycemia. Lancet 2, 13481351 (1962)

  • 27.

    Kurzer MS , Xu X: Dietary phytoestrogens. Annu Rev Nu 17, 353381 (1997)

  • 28.

    Axelson M , Sjövall J, Gustafsson BE, Setchell KD: Soya –a dietary source of the nonsteroidal oestrogen equol in man and animals. Endocrinol 102, 4956 (1984)

    • Search Google Scholar
    • Export Citation
  • Collapse
  • Expand

2019  
Scimago
H-index
11
Scimago
Journal Rank
0,220
Scimago
Quartile Score
Medicine (miscellaneous) Q3
Scopus
Cite Score
155/133=1,2
Scopus
Cite Score Rank
General Medicine 199/529 (Q2)
Scopus
SNIP
0,343
Scopus
Cites
206
Scopus
Documents
23

 

Interventional Medicine and Applied Science
Language English
Size  
Year of
Foundation
2009
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2061-1617 (Print)
ISSN 2061-5094 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2022 0 24 9
Jul 2022 0 21 17
Aug 2022 0 28 10
Sep 2022 0 25 16
Oct 2022 0 18 15
Nov 2022 0 21 22
Dec 2022 0 0 0